Isoforms of acyl-CoA:diacylglycerol acyltransferase2 differ substantially in their specificities toward erucic acid

Kamil Demski , Simon Jeppson , Ida Lager , Agnieszka Misztak , Katarzyna Jasieniecka-Gazarkiewicz , Małgorzata Waleron , Sten Stymne , Antoni Banaś

Abstract

In most oilseeds, two evolutionarily unrelated acyl-CoA:diacylglycerol acyltransferase (DGAT) enzymes, DGAT1 and DGAT2, are the main contributors to the acylation of diacylglycerols in the synthesis of triacylglycerol. DGAT1 and DGAT2 are both present in the important crop oilseed rape (Brassica napus), with each type having four isoforms. We studied the activities of DGAT isoforms during seed development in microsomal fractions from two oilseed rape cultivars: edible, low-erucic acid (22:1) MONOLIT and nonedible high-erucic acid MAPLUS. Whereas the specific activities of DGATs were similar with most of the testedacyl-CoAsubstratesinbothcultivars,MAPLUShad6-to14-foldhigheractivitywith22:1-CoAthandidMONOLIT.Thus, DGAT isoforms with different acyl-CoA specificities are differentially active in the two cultivars. We characterized the acyl-CoA specificities of all DGAT isoforms in oilseed rape in the microsomal fractions of yeast cells heterologously expressing these enzymes. All four DGAT1 isoforms showed similar and broad acyl-CoA specificities. However, DGAT2 isoforms had much narrower acyl-CoA specificities: two DGAT2 isoforms were highly active with 22:1-CoA, while the ability of the other two isoforms to use this substrate was impaired. These findings elucidate the importance, which a DGAT isoform with suitable acylCoA specificity may have, when aiming for high content of a particular fatty acid in plant triacylglycerol reservoirs.
Author Kamil Demski (IFB / IB / DBiotech)
Kamil Demski,,
- Department of Biotechnology
, Simon Jeppson
Simon Jeppson,,
-
, Ida Lager
Ida Lager,,
-
, Agnieszka Misztak (IFB)
Agnieszka Misztak,,
- Intercollegiate Faculty of Biotechnology UG
, Katarzyna Jasieniecka-Gazarkiewicz (IFB / IB / DBiotech)
Katarzyna Jasieniecka-Gazarkiewicz,,
- Department of Biotechnology
, Małgorzata Waleron (IFB / IB / DBiotech)
Małgorzata Waleron,,
- Department of Biotechnology
, Sten Stymne
Sten Stymne,,
-
, Antoni Banaś (IFB / IB / DBiotech)
Antoni Banaś,,
- Department of Biotechnology
Journal seriesPlant Physiology, ISSN 0032-0889, e-ISSN 1532-2548, (N/A 140 pkt)
Issue year2019
Vol181
No4
Pages1468-1479
Publication size in sheets0.55
ASJC Classification1110 Plant Science; 1311 Genetics; 1314 Physiology
DOIDOI:10.1104/pp.19.01129
URL https://doi.org/10.1104/pp.19.01129
Languageen angielski
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 28-01-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.598; WoS Impact Factor: 2018 = 6.305 (2) - 2018=7.024 (5)
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