Permeability testing of the vitamins A + E complex contained in emulsions using a Franz diffusion cell

B. Łubkowska , M. Hus , Beata Grobelna , Zbigniew Maćkiewicz


Objective — to test whether active substances, such as vitamin A and vitamin E, contained in emulsion will penetrate the membrane imitating skin.Materials and methods. Two derivatives were the analysed active compounds: retinol palmitate and tocopherol acetate. Permeation studies were conducted using Franz-type diffusion cells made of borosilicate glass. The collected samples were subjected to determination by the MALDI-TOF MS method and the analytical HPLC technique. The studies were in vitro tests, which allow for testing of active substances outside a living organism while representing the effects of these compounds on the organism.Results and discussion. The permeability testing shows that some of the compounds contained in the w/o type of emulsion pass through membranes, which was confirmed using selected methods of HPLC qualitative analysis and MALDI-TOF MS. In addition, apart from the analysed complex, other active ingredients included in the emulsion formula also managed to get through.Conclusions. In the test carried out at a temperature of (37 ± 0.5) °C in a diffusion cell derivatives of vitamins A and E were detected much faster than in the tests carried out at laboratory temperature of (22 ± 0.5) °C. The temperature of 37 °C corresponds to the transdermal systems used on the skin.
Author B. Łubkowska
B. Łubkowska,,
, M. Hus
M. Hus,,
, Beata Grobelna (FCh / DACh / LChAC)
Beata Grobelna,,
- Laboratory of chemistry and Analytics of Cosmetics
, Zbigniew Maćkiewicz (FCh / DMBt / LChBM)
Zbigniew Maćkiewicz,,
- Laboratory of Chemistry of Biological Macromolecules
Journal seriesUkrainian Journal of Dermatology, Venerology, Cosmetology, ISSN 1727-5741, (0 pkt)
Issue year2017
No1 (64)
Publication size in sheets0.72
Keywords in EnglishVitamin A, vitamin E, in vitro permeability, Franz diffusion cell
Languageen angielski
Score (nominal)5
Score sourcejournalList
ScoreMinisterial score = 5.0, 28-01-2020, ArticleFromJournal
Citation count*1 (2020-05-20)
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