Lack of intracellular trehalose affects formation of Escherichia coli persister cells
Dorota Kuczyńska-Wiśnik , Karolina Stojowska , Ewelina Matuszewska , Daria Leszczyńska , Maria Moruno Algara , Mateusz Augustynowicz , Ewa Laskowska
AbstractPersisters are dormant antibiotic-tolerant cells that usually compose a small fraction of bacterial populations. In this work, we focused on the role of trehalose in persister formation. We found that the ΔotsA mutant, which is unable to synthesize trehalose, produced increased levels of persisters in the early stationary phase and under heat stress conditions. The lack of trehalose in the ΔotsA mutant resulted in oxidative stress, manifested by increased membrane lipid peroxidation after heat shock. Stationary ΔotsA cells additionally exhibited increased levels of oxidized proteins and apurinic/apyrimidinic sites in DNA as compared to WT cells. Oxidative stress caused by the lack of trehalose was accompanied by the overproduction of extracellular indole, a signal molecule that has been shown to stimulate persister formation. Our major conclusion is that intracellular trehalose protects E. coli cells against oxidative stress and limits indole synthesis, which in turn inhibits the formation of persisters.
|Journal series||Microbiology-Sgm, ISSN 1350-0872, (A 30 pkt)|
|Publication size in sheets||0.50|
|Keywords in English||Escherichia coli, trehalose, antibiotic tolerance, persister cells|
|Score|| = 30.0, 24-07-2019, ArticleFromJournal|
= 30.0, 24-07-2019, ArticleFromJournal
|Publication indicators||: 2015 = 0.834; : 2015 = 2.268 (2) - 2015=2.879 (5)|
|Citation count*||17 (2020-06-19)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.