Lack of intracellular trehalose affects formation of Escherichia coli persister cells

Dorota Kuczyńska-Wiśnik , Karolina Stojowska , Ewelina Matuszewska , Daria Leszczyńska , Maria Moruno Algara , Mateusz Augustynowicz , Ewa Laskowska

Abstract

Persisters are dormant antibiotic-tolerant cells that usually compose a small fraction of bacterial populations. In this work, we focused on the role of trehalose in persister formation. We found that the ΔotsA mutant, which is unable to synthesize trehalose, produced increased levels of persisters in the early stationary phase and under heat stress conditions. The lack of trehalose in the ΔotsA mutant resulted in oxidative stress, manifested by increased membrane lipid peroxidation after heat shock. Stationary ΔotsA cells additionally exhibited increased levels of oxidized proteins and apurinic/apyrimidinic sites in DNA as compared to WT cells. Oxidative stress caused by the lack of trehalose was accompanied by the overproduction of extracellular indole, a signal molecule that has been shown to stimulate persister formation. Our major conclusion is that intracellular trehalose protects E. coli cells against oxidative stress and limits indole synthesis, which in turn inhibits the formation of persisters.
Author Dorota Kuczyńska-Wiśnik (FB / DB)
Dorota Kuczyńska-Wiśnik,,
- Department of Biochemistry
, Karolina Stojowska (FB / DB)
Karolina Stojowska,,
- Department of Biochemistry
, Ewelina Matuszewska (FB / DB)
Ewelina Matuszewska,,
- Department of Biochemistry
, Daria Leszczyńska (FB / DB)
Daria Leszczyńska,,
- Department of Biochemistry
, Maria Moruno Algara (FB / DB)
Maria Moruno Algara,,
- Department of Biochemistry
, Mateusz Augustynowicz (FB / DB)
Mateusz Augustynowicz ,,
- Department of Biochemistry
, Ewa Laskowska (FB / DB)
Ewa Laskowska,,
- Department of Biochemistry
Journal seriesMicrobiology-Sgm, ISSN 1350-0872, (A 25 pkt)
Issue year2015
Vol161
No4
Pages786-796
Publication size in sheets0.5
Keywords in EnglishEscherichia coli, trehalose, antibiotic tolerance, persister cells
DOIDOI:10.1099/mic.0.000012
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, ArticleFromJournal
Publication indicators WoS Impact Factor: 2015 = 2.268 (2) - 2015=2.879 (5)
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