Effect of 11β-hydroxyandrostenedione on European whitefish Coregonus lavaretus (Linnaeus, 1758)

Jarosław Król , Piotr Hliwa , Adam Polewacz , Agnieszka Stabińska , Stefan Dobosz , Konrad Ocalewicz

Abstract

The goal of the present research was to evaluate the efficiency of 11β-hydroxyandrostenedione (OHA) applied in the diet to achieve sex reversal in the European whitefish (Coregonus lavaretus). At 32 day post-hatching, fish were reared in four groups: fish fed with 10 ppm of OHA (10 OHA), fish fed with 20 ppm of OHA (20 OHA), fish fed without OHA (C) and fish fed without OHA and reared in the water from 20 OHA group (R). The experimental groups were conducted in separate recirculation systems and the first phase of the experiment lasted 63 days. For the histological analysis of the gonads, fish from all groups were reared without OHA treatment for an additional 91 days (second phase). At the end of the first phase of the experiment, survival of the whitefish ranged from 34.5 ± 11.1% to 51.5 ± 7.3%. The final body weight and coefficient of variation in the weight ranged from 5.6 ± 1.2 g to 6.9 ± 1.5 g and from 21.5 to 22.7%, respectively. No negative effects of OHA treatment on the growth and the survival of the whitefish were found. Six histological categories of the whitefish gonads were observed. Apart from the typical ovaries and testes, two types of the intersexual gonads (ovotestis and testis-ova) and two types of the sterile-altered gonads were distinguished. No gonadal females were found among fish from any of OHA groups. Gonadal males constituted of 60% and 50% of the fish from 10 OHA and 20 OHA groups, respectively. Intersexes were observed in all groups with the highest proportion found among fish from R variant. Rate of sterile individuals in 10 OHA and 20 OHA groups was 17% and 30%, respectively. The proportion of fish with normal testes to fish with other types of gonad varied from 0.43:1 to 1.5:1 with the higher ratio observed in both OHA groups. Lack of the females among fish from OHA groups suggested OHA affected growth and development of ovaries in the whitefish. However, a high percentage of the sterile fish in both OHA treated groups indicated application of lower doses of OHA for masculinization of the whitefish in the further research.
Author Jarosław Król
Jarosław Król,,
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, Piotr Hliwa
Piotr Hliwa,,
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, Adam Polewacz
Adam Polewacz,,
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, Agnieszka Stabińska
Agnieszka Stabińska,,
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, Stefan Dobosz
Stefan Dobosz,,
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, Konrad Ocalewicz ZBiEM
Konrad Ocalewicz,,
- Department of Marine Biology and Ecology
Other language title versions
Journal seriesAnimal Reproduction Science, ISSN 0378-4320
Issue year2017
Vol184
Pages187-195
Publication size in sheets0.5
Keywords in Englishwhitefish, sex differentiation, masculinization, OHA, intersexual gonads
DOIDOI:10.1016/j.anireprosci.2017.07.015
URL https://doi.org/10.1016/j.anireprosci.2017.07.015
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.605 (2) - 2016=1.793 (5)
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