Characterization of a bacteriophage, vB_Eco4M-7, that effectively infects many Escherichia coli O157 strains

Agnieszka Necel , Sylwia Bloch , Bożena Nejman-Faleńczyk , Michał Grabski , Gracja Topka , Aleksandra Dydecka , Katarzyna Kosznik-Kwaśnicka , Łukasz Grabowski , Agata Jurczak-Kurek , Tomasz Wołkowicz , Grzegorz Węgrzyn , Alicja Węgrzyn

Abstract

The characterization of a recently isolated bacteriophage, vB_Eco4M-7, which efectively infects many, though not all, Escherichia coli O157 strains, is presented. The genome of this phage comprises doublestranded DNA, 68,084bpin length, with a GC content of 46.2%. It contains 96 putative open reading frames (ORFs). Among them, the putative functions of only 35 ORFs were predicted (36.5%), whereas 61 ORFs (63.5%) were classifed as hypothetical proteins. The genome of phage vB_Eco4M-7 does not contain genes coding for integrase, recombinase, repressors or excisionase, which are the main markers of temperate viruses. Therefore, we conclude that phage vB_Eco4M-7 should be considered a lytic virus. This was confrmed by monitoring phage lytic development by a one-step growth experiment. Moreover, the phage forms relatively small uniform plaques (1mm diameter) with no properties of lysogenization. Electron microscopic analyses indicated that vB_Eco4M-7 belongs to the Myoviridae family. Based on mass spectrometric analyses, including the fragmentation pattern of unique peptides, 33 phage vB_Eco4M-7 proteins were assigned to annotated open reading frames. Importantly, genome analysis suggested that this E. coli phage is free of toxins and other virulence factors. In addition, a similar, previously reported but uncharacterized bacteriophage, ECML-117, was also investigated, and this phage exhibited properties similar to vB_Eco4M-7. Our results indicate that both studied phages are potential candidates for phage therapy and/or food protection against Shiga toxin-producing E. coli, as the majority of these strains belong to the O157 serotype.
Author Agnieszka Necel (FB/DMoB)
Agnieszka Necel,,
- Department of Molecular Biology
, Sylwia Bloch - [Polish Academy of Science]
Sylwia Bloch,,
-
- Polska Akademia Nauk
, Bożena Nejman-Faleńczyk (FB/DMoB)
Bożena Nejman-Faleńczyk,,
- Department of Molecular Biology
, Michał Grabski (FB/DMoB)
Michał Grabski,,
- Department of Molecular Biology
, Gracja Topka (FB/DMoB)
Gracja Topka,,
- Department of Molecular Biology
, Aleksandra Dydecka (FB/DMoB)
Aleksandra Dydecka ,,
- Department of Molecular Biology
, Katarzyna Kosznik-Kwaśnicka - [Polish Academy of Science]
Katarzyna Kosznik-Kwaśnicka ,,
-
- Polska Akademia Nauk
, Łukasz Grabowski
Łukasz Grabowski,,
-
, Agata Jurczak-Kurek (FB/DME)
Agata Jurczak-Kurek,,
- Department of Molecular Evolution
, Tomasz Wołkowicz
Tomasz Wołkowicz,,
-
et al.`
Journal seriesScientific Reports, ISSN 2045-2322, (N/A 140 pkt)
Issue year2020
Vol10
Pages1-14
Publication size in sheets0.65
Article number3743
ASJC Classification1000 Multidisciplinary
DOIDOI:10.1038/s41598-020-60568-4
URL https://doi.org/10.1038/s41598-020-60568-4
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 09-03-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.401; WoS Impact Factor: 2018 = 4.011 (2) - 2018=4.525 (5)
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