Antimicrobial blue light photoinactivation of Pseudomonas aeruginosa: quorum sensing signaling molecules, biofilm formation and pathogenicity

Grzegorz Fila , Marta Krychowiak , Michał Rychłowski , Krzysztof Piotr Bielawski , Mariusz Grinholc

Abstract

Pseudomonas aeruginosa is a common causative bacterium of acute and chronic infections that have been responsible for high mortality over the past decade. P. aeruginosa produces many virulence factors such as toxins, enzymes and dyes that are strongly dependent on quorum sensing (QS) signaling systems. P. aeruginosa has three major QS systems (las, rhl and Pseudomonas quinolone signal) that regulate the expression of genes encoding virulence factors as well as biofilm production and maturation. Antimicrobial blue light (aBL) is considered a therapeutic option for bacterial infections and has other benefits, such as reducing bacterial virulence. Therefore, this study investigated the efficacy of aBL to reduce P. aeruginosa pathogenicity. aBL treatment resulted in the reduced activity of certain QS signaling molecules in P. aeruginosa and inhibited biofilm formation. in vivo tests using a Caenorhabditis elegans infection model indicated that sublethal aBL decreased the pathogenicity of P. aeruginosa. aBL may be a new virulence-targeting therapeutic approach.
Author Grzegorz Fila (IFB)
Grzegorz Fila,,
- Intercollegiate Faculty of Biotechnology UG
, Marta Krychowiak (IFB)
Marta Krychowiak,,
- Intercollegiate Faculty of Biotechnology UG
, Michał Rychłowski (IFB / IB / LVMB)
Michał Rychłowski,,
- Laboratory of Virus Molecular Biology
, Krzysztof Piotr Bielawski (IFB / IB / DBiotech)
Krzysztof Piotr Bielawski,,
- Department of Biotechnology
, Mariusz Grinholc (IFB / IB / DBiotech)
Mariusz Grinholc,,
- Department of Biotechnology
Journal seriesJournal of Biophotonics, ISSN 1864-063X, (A 35 pkt)
Issue year2018
Vol11
No11
Pages1-12
Publication size in sheets0.55
Keywords in Englishantimicrobial blue light, biofilm, Caenorhabditis elegans, photodynamic inactivation, photoinactivation, Pseudomonas aeruginosa, quorum sensing
ASJC Classification3100 General Physics and Astronomy; 2200 General Engineering; 1300 General Biochemistry, Genetics and Molecular Biology; 2500 General Materials Science; 1600 General Chemistry
DOIDOI:10.1002/jbio.201800079
URL https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.201800079
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)35
ScoreMinisterial score = 35.0, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, ArticleFromJournal
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.212; WoS Impact Factor: 2017 = 3.768 (2) - 2017=3.694 (5)
Citation count*2 (2019-04-18)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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