Suitability of selected chromatographic columns for analysis of fatty acids in dialyzed patients

Magdalena Pazda , Piotr Stepnowski , Tomasz Śledziński , Michał Chmielewski , Adriana Mika

Abstract

Gas chromatography–mass spectrometry is a preferred method for fatty acid (FA) analysis in biofluids from patients with metabolic diseases. Complex characteristics of FAs make their analysis particularly challenging. Selection of an appropriate chromatographic column is particularlyimportant component of the process as it provides optimal separation and detection of possibly all FAs present in the sample. However, no accurate protocol for comparative evaluation of capillary columns for the analysis of whole serum FA profile in patients with chronic kidney disease(CKD) has been developed thus far. Therefore, in the present study four columns were examined to select the one providing optimal separation and determination of FA profiles in this group of patients. Moreover, serum FA profiles obtained with the selected column in CKD patients subjected to peritoneal dialysis and healthy controls were compared. Thirty‐seven component FAME Mix and sera from CKD patients were used to optimize chromatographic conditions and to select the most appropriate column. The ZB‐5 column turned out to be the most appropriate for the analysis of whole FA profile in CKD patients' sera. Then, this column was used to compare FAprofiles in patients subjected to peritoneal dialysis and in healthy controls. The analysis demon-strated many abnormalities in the FA profile of CKD patients. Further studies involving larger groups of patients presenting with other stages of CKD are required to explain the impact ofthe disease progression on composition of serum FAs.
Author Magdalena Pazda PAiDCh
Magdalena Pazda,,
- Laboratory of Chemical Analytics and Diagnostics
, Piotr Stepnowski PChZŚ
Piotr Stepnowski,,
- Laboratory of Chemical Environmental Risks
, Tomasz Śledziński
Tomasz Śledziński,,
-
, Michał Chmielewski
Michał Chmielewski,,
-
, Adriana Mika PChZŚ
Adriana Mika,,
- Laboratory of Chemical Environmental Risks
Other language title versions
Journal seriesBiomedical Chromatography, ISSN 0269-3879
Issue year2017
Vol31
No11
Pages1-8
Publication size in sheets0.5
Keywords in Englishchromatographic column, chronic kidney disease, fatty acid, gas chromatography-mass spectrometry, peritoneal dialysis
DOIDOI:10.1002/bmc.4006
URL https://doi.org/10.1002/bmc.4006
Languageen angielski
Score (nominal)20
ScoreMinisterial score = 20.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 20.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.613 (2) - 2016=1.612 (5)
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