Pectobacterium zantedeschiae sp. nov. a new species of a soft rot pathogen isolated from Calla lily (Zantedeschia spp.)

Małgorzata Waleron , Agnieszka Misztak , Michał Waleron , Martyna Franczuk , Joanna Jońca , Bartosz Wielgomas , Artur Mikiciński , Tatjana Popović , Krzysztof Waleron

Abstract

Four Gram-negative, rod-shaped pectinolytic bacterial strains designated as 2M, 9M, DPMP599 and DPMP600 were subjected to polyphasic analyses that revealed their distinctiveness from the other Pectobacterium species. Strains 2M and 9M were isolated from Calla lily bulbs cultivated in Central Poland. DPMP599 and DPMP600 strains were isolated from Calla lily leaves from plants grown in Serbia. Phylogenetic analyses based on nine housekeeping genes (gapA, gyrA, icdA, pgi, proA, recA, recN, rpoA, and rpoS), as well as phylogeny based on the 381 most conserved universal proteins confirmed that Pectobacterium zantedeschiae strains were distantly related to the other Pectobacterium, and indicated Pectobacterium atrosepticum, Pectobacterium betavasculorum, Pectobacterium parmentieri and Pectobacterium wasabiae as the closest relatives. Moreover, the analysis revealed that Pectobacterium zantedeschiae strains are not akin to Pectobacterium aroidearum strains, which were likewise isolated from Calla lily. The genome sequencing of the strains 2M, 9M and DPMP600 and their comparison with whole genome sequences of other Pectobacterium type strains confirmed their distinctiveness and separate species status within the genus based on parameters of in silico DNA–DNA hybridization and average nucleotide identity (ANI) values. The MALDI-TOF MS proteomic profile supported the proposition of delineation of the P. zantedeschiae and additionally confirmed the individuality of the studied strains. Based on of all of these data, it is proposed that the strains 2M, 9M, DPMP599, and DPMP600 isolated from Calla lily, previously assigned as P. atrosepticum should be reclassified as Pectobacterium zantedeschiae sp. nov. with the strain 9MT (PCM2893 = DSM105717 = IFB9009) as the type strain.
Author Małgorzata Waleron (IFB / IB / DBiotech)
Małgorzata Waleron,,
- Department of Biotechnology
, Agnieszka Misztak (IFB)
Agnieszka Misztak,,
- Intercollegiate Faculty of Biotechnology UG
, Michał Waleron (IFB)
Michał Waleron,,
- Intercollegiate Faculty of Biotechnology UG
, Martyna Franczuk
Martyna Franczuk,,
-
, Joanna Jońca
Joanna Jońca,,
-
, Bartosz Wielgomas
Bartosz Wielgomas,,
-
, Artur Mikiciński
Artur Mikiciński,,
-
, Tatjana Popović
Tatjana Popović,,
-
, Krzysztof Waleron
Krzysztof Waleron,,
-
Journal seriesSystematic and Applied Microbiology, ISSN 0723-2020, (A 30 pkt)
Issue year2019
Vol42
No3
Pages275-283
Publication size in sheets0.5
Keywords in EnglishMLSA, ANI, isDNA-DNA hybridization, FAME, MALDI-TOF MS
ASJC Classification2402 Applied Microbiology and Biotechnology; 1105 Ecology, Evolution, Behavior and Systematics; 2404 Microbiology
DOIDOI:10.1016/j.syapm.2018.08.004
URL https://doi.org/10.1016/j.syapm.2018.08.004
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 24-07-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.368; WoS Impact Factor: 2017 = 3.899 (2) - 2017=3.644 (5)
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