Molecular methods as tools to control plant diseases caused by Dickeya and Pectobacterium spp: A minireview

Agata Motyka , Sabina Żołędowska , Wojciech Śledź , Ewa Łojkowska


Dickeya spp. and Pectobacterium spp. are etiological agents of soft rot on crops, vegetables, and ornamentals. They also cause blackleg on potato. These pectinolytic phytopathogens are responsible for significant economic losses, mostly within the potato production sector. Importantly, there are no methods to eradicate these microorganisms once they have infected plant material. Solely preventive measures remain, including early detection and identification of the pathogens, monitoring of their spread in addition to planting certified seed material tested for latent infections. As proper identification of the causative agent allows for efficient limitation of disease spread, numerous detection and differentiation methods have been developed. Most commonly followed procedures involve: isolation of viable bacterial cells (alternatively post-enrichment) on semi-selective media, identification to species level by PCR (single, multiplex, Real time), serology or fatty acids profiling. Differentiation of the isolates is often accomplished by sequencing the housekeeping genes or molecular fingerprinting. In view of lowering total costs of next-generation sequencing (NGS), a huge amount of generated data reveals subtle differences between strains that have proven to be potentially useful for the establishment of specific novel detection pipelines. Successful implementation of molecular diagnostic methods is exemplified by 20-year studies on the populations of pectinolytic bacteria on potatoes in Poland. The presented work aims to gather the characteristics of Dickeya spp. and Pectobacterium spp. important for the identification process in addition to providing an overview of modern and newly developed specific, rapid, high-throughput and cost-effective screening methods for the detection and identification of these phytopathogens.
Author Agata Motyka (IFB)
Agata Motyka,,
- Intercollegiate Faculty of Biotechnology UG
, Sabina Żołędowska (IFB)
Sabina Żołędowska,,
- Intercollegiate Faculty of Biotechnology UG
, Wojciech Śledź (IFB / IB / DBiotech)
Wojciech Śledź,,
- Department of Biotechnology
, Ewa Łojkowska (IFB / IB / DBiotech)
Ewa Łojkowska,,
- Department of Biotechnology
Journal seriesNew Biotechnology, ISSN 1871-6784, (A 30 pkt)
Issue year2017
Publication size in sheets0.5
Keywords in EnglishDetection, differentiation, Erwinia, identification, pectinolytic bacteria, plant protection
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.813 (2) - 2016=3.24 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.