Molecular action of isoflavone genistein in the human epithelial cell line HaCaT

Elwira Smolińska , Marta Moskot , Joanna Jakóbkiewicz-Banecka , Grzegorz Węgrzyn , Bogdan Banecki , Aneta Szczerkowska-Dobosz , Dorota Purzycka-Bohdan , Magdalena Gabig-Cimińska


Due to its strong proliferation-reducing effects on keratinocytes, and also anti-inflammatory properties, the isoflavone genistein has already been proposed as a possible antipsoriatic compound. As there is still no detailed information on this topic, we examined the effects of genistein by using an in vitro model of both, normal and ªpsoriasis-likeº keratinocytes at this stage of our work exhaustively testing the selected flavonoid in a mono-treated experimental design. Gene expression studies revealed transcriptional changes that confirms known disease- associated pathways and highlights many psoriasis-related genes. Our results suggested that aberrant expression of genes contributing to the progress of psoriasis could be improved by the action of genistein. Genistein prevented ªcytokine mixº as well as TNF-α- induced NF-κB nuclear translocation, with no effect on the PI3K signaling cascade, indicating the luck of turning this pathway into NF-κB activation. It could have attenuated TNF-α and LPS-induced inflammatory responses by suppressing ROS activation. Regardless of the type of keratinocyte stimulation used, reduction of cytokine IL-8, IL-20 and CCL2 production (both at RNA and protein level) following genistein treatment was visible. Because investigations of other groups supported our commentary on potential administration of genistein as a potential weapon in the armamentarium against psoriasis, it is believed that this paper should serve to encourage researchers to conduct further studies on this subject.
Author Elwira Smolińska (FB / DMoB) - [Medical Uniwersity of Gdańsk (GUMed)]
Elwira Smolińska ,,
- Department of Molecular Biology
- Gdański Uniwersytet Medyczny
, Marta Moskot (FB / DMoB)
Marta Moskot,,
- Department of Molecular Biology
, Joanna Jakóbkiewicz-Banecka (FB / DMoB)
Joanna Jakóbkiewicz-Banecka,,
- Department of Molecular Biology
, Grzegorz Węgrzyn (FB / DMoB)
Grzegorz Węgrzyn,,
- Department of Molecular Biology
, Bogdan Banecki (IFB / M020 / DMCB)
Bogdan Banecki,,
- Department of Molecular and Cellular Biology
, Aneta Szczerkowska-Dobosz - [Gdanski Uniwersytet Medyczny]
Aneta Szczerkowska-Dobosz,,
- Gdanski Uniwersytet Medyczny
, Dorota Purzycka-Bohdan - [Gdanski Uniwersytet Medyczny]
Dorota Purzycka-Bohdan,,
- Gdanski Uniwersytet Medyczny
, Magdalena Gabig-Cimińska (FB / DMBG) - [Polish Academy of Science]
Magdalena Gabig-Cimińska,,
- Katedra Biologii i Genetyki Medycznej
- Polska Akademia Nauk
Journal seriesPLoS ONE, ISSN 1932-6203, (A 40 pkt)
Issue year2018
Publication size in sheets1.3
Article numbere0192297
ASJC Classification2700 General Medicine; 1100 General Agricultural and Biological Sciences; 1300 General Biochemistry, Genetics and Molecular Biology
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 29-05-2020, ArticleFromJournal
Publication indicators WoS Citations = 3; Scopus Citations = 8; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.111; WoS Impact Factor: 2018 = 2.776 (2) - 2018=3.337 (5)
Citation count*9 (2020-05-12)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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