Insights into cyanophage-mediated dynamics of nodularin and other non-ribosomal peptides in Nodularia spumigena

Sigitas Šulčius , Hanna Mazur-Marzec , Irma Vitonytė , Kotryna Kvederavičiūtė , Jolita Kuznecova , Eugenijus Šimoliūnas , Karin Holmfeldt

Abstract

The effect of cyanophage infection and lysis on the dynamics of the hepatotoxin nodularin (NOD) and other non-ribosomal peptides (NRPs) produced by cyanobacteria is poorly understood. In this study, changes in concentration of NOD and other NRPs during cyanophage infection of the filamentous cyanobacteria Nodularia spumigena were assessed using incubation experiments. Viral infection and lysis were associated with a significant reduction (93% at the 96 h post infection) of N. spumigena cell density. While no correlation between N. spumigena abundance and total concentration of NOD (ng mL−1) within the infected cells was observed, cellular NOD quota (ng cell−1) gradually increased in the remaining cyanophage resistant N. spumigena subpopulation. Lysis of N. spumigena cells resulted in a substantial increase (>57 times) of dissolved NOD concentration in the culture medium. The relative concentration of other cyclic (anabaenopeptins) and linear (aeruginosins, spumigins) NRPs produced by N. spumigena also increased in response to cyanophage addition. This study highlights the importance of cyanophage infection on the population toxicity of filamentous cyanobacteria and demonstrates a significant contribution of virus-mediated cell lysis on the conversion of NOD from the particulate to dissolved phase.
Author Sigitas Šulčius
Sigitas Šulčius,,
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, Hanna Mazur-Marzec ZBM
Hanna Mazur-Marzec,,
- Division of Marine Biotechnology
, Irma Vitonytė
Irma Vitonytė,,
-
, Kotryna Kvederavičiūtė
Kotryna Kvederavičiūtė,,
-
, Jolita Kuznecova
Jolita Kuznecova,,
-
, Eugenijus Šimoliūnas
Eugenijus Šimoliūnas,,
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, Karin Holmfeldt
Karin Holmfeldt,,
-
Journal seriesHarmful Algae, ISSN 1568-9883
Issue year2018
Vol78
Pages69-74
Publication size in sheets0.5
Keywords in EnglishAquatic viruses, Baltic Sea, cyanotoxins, filamentous cyanobacteria, virus-bacterium interactions
DOIDOI:10.1016/j.hal.2018.07.004
URL http://doi.org/10.1016/j.hal.2018.07.004
Languageen angielski
Score (nominal)45
ScoreMinisterial score = 40.0, 21-08-2018, ArticleFromJournal
Ministerial score (2013-2016) = 45.0, 21-08-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.087 (2) - 2016=3.539 (5)
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