Synthesis of silver nanoparticles in context of their cytotoxicity, antibacterial activities, skin penetration and application in skincare products

Elżbieta Szczepańska , Aleksandra Bielicka-Giełdoń , Karolina Niska , Justyna Strankowska , Joanna Żebrowska , Iwona Inkielewicz-Stepniak , Beata Łubkowska , Tomasz Swebocki , Piotr Skowron , Beata Grobelna


The benefits of silver nanoparticles (AgNPs) have been known in many fields from catalysis through optics to medicine due to their unique size-dependent properties. Recently, there is also a growing interest in novel skincare formulations containing AgNPs. In this work, AgNPs were successfully synthesised and were characterised by UV-Vis spectroscopy and Transmission Electron Microscopy (TEM). In addition, an analysis of particle size distribution (measured by Dynamic Light Scattering – DLS) and zeta potential (using the method Electrophoretic Light Scattering – ELS) were made. The obtained AgNPs were in two sizes: small (20 nm ± 5 nm) and large (100 nm ± 5 nm). The assessment of the cytotoxic effect on human skin cells was done. Smaller AgNPs showed less cytotoxicity and thus greater safety for skin, so they were introduced into new skincare formulations such as oil/water (O/W) emulsion, water/oil (W/O) emulsion and hydrogel. Moreover, the study of release AgNPs from emulsions and hydrogel using Franz diffusion chamber with pig skin as a membrane has been conducted. The receptor fluid measurements were performed by Atomic Absorption Spectroscopy (AAS). The last point of the research was the performance of microbiological tests to determine the antibacterial effect of the smaller AgNPs in both solution and cosmetic.
Publication typeIn press (online first, early view)
Author Elżbieta Szczepańska (FCh/DACh/LChAC)
Elżbieta Szczepańska,,
- Laboratory of chemistry and Analytics of Cosmetics
, Aleksandra Bielicka-Giełdoń (FCh/DET/LAOP)
Aleksandra Bielicka-Giełdoń,,
- Laboratory of Advanced Oxidation Processes
, Karolina Niska
Karolina Niska,,
, Justyna Strankowska (FMPI/IEP)
Justyna Strankowska,,
- Institute of Experimental Physics
, Joanna Żebrowska (FCh/DMBt/LGE)
Joanna Żebrowska,,
- Laboratory of Genetic Engineering
, Iwona Inkielewicz-Stepniak
Iwona Inkielewicz-Stepniak,,
, Beata Łubkowska (FCh/DMBt/LChBM)
Beata Łubkowska,,
- Laboratory of Chemistry of Biological Macromolecules
, Tomasz Swebocki (FCh/DACh/LSCh)
Tomasz Swebocki,,
- Laboratory of Supramolecular Chemistry
, Piotr Skowron (FCh/DMBt/LGE)
Piotr Skowron,,
- Laboratory of Genetic Engineering
, Beata Grobelna (FCh/DACh/LChAC)
Beata Grobelna,,
- Laboratory of chemistry and Analytics of Cosmetics
Journal seriesSupramolecular Chemistry, ISSN 1061-0278, e-ISSN 1029-0478, (N/A 40 pkt)
Issue year2020
Noonline first
Publication size in sheets0.70
Keywords in EnglishSilver nanoparticles, Franz diffusion cell, in vitro penetration of Ago nanoparticles, anti-microbiological activity, cytotoxic effect
ASJC Classification1600 General Chemistry
Languageen angielski
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 21-03-2020, ArticleFromJournal
Publication indicators WoS Citations = 0.000; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.374; WoS Impact Factor: 2018 = 1.660 (2) - 2018=1.313 (5)
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