Comparison of three extraction approaches for the isolation of neurotransmitters from rat brain samples

Natalia Miękus , Ilona Olędzka , Darya Harshkova , Ivan Liakh , Alina Plenis , Piotr Kowalski , Tomasz Bączek

Abstract

The determination of neurotransmitters (NTs) as relevant potential biomarkers in the study of various central nervous system (CNS) pathologies has been demonstrated. Knowing that NTs-related diseases mostly occupy individual regions of the nervous system, as observed, for instance, in neurodegenerative diseases (Alzheimer’s and Parkinson’s Diseases), the analysis of brain slices is preferred to whole-brain analysis. In this report, we present sample preparation approaches, such as solid-phase extraction, solid-phase microextraction, and dispersive liquid–liquid microextraction, and discuss the pitfalls and advantages of each extraction method. The ionic liquid (1-ethyl-3-methylimidazolium tetrafluoroborate)-assisted solid-phase microextraction (IL-SPME) is found to be, in our research, the relevant step towards the simultaneous determination of six NTs, namely, dopamine (DA), adrenaline (A), noradrenaline (NA), serotonin (5-HT), L-tryptophan (L-Trp), L-tyrosine (L-Tyr) in rat brain samples. The development of a novel bioanalytical technique for the evaluation of biomarkers in the context of green chemistry might be accelerated just with the use of IL, and this approach can be considered an advantageous strategy.
Author Natalia Miękus KFZiC
Natalia Miękus,,
- Department of Animal and Human Physiology
, Ilona Olędzka
Ilona Olędzka,,
-
, Darya Harshkova KFiBR
Darya Harshkova,,
- Department of Plant Physiology and Biotechnology
, Ivan Liakh
Ivan Liakh,,
-
, Alina Plenis
Alina Plenis,,
-
, Piotr Kowalski
Piotr Kowalski,,
-
, Tomasz Bączek
Tomasz Bączek,,
-
Journal seriesInternational Journal of Molecular Sciences, ISSN 1661-6596, e-ISSN 1422-0067
Issue year2018
Vol19
No6
Pages1-11
Publication size in sheets0.5
Keywords in Englishbrain cortex slices, neurotransmitters, micellar electrokinetic capillary chromatography
DOIDOI:10.3390/ijms19061560
URL https://doi.org/10.3390/ijms19061560
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)30
ScoreMinisterial score = 30.0, 28-05-2018, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 28-05-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.226 (2) - 2016=3.482 (5)
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