Copper(II) coordination properties of GxG peptides: key role of sidechains of central residues on coordination of formed systems; combined potentiometric and ITC studies
Joanna Makowska , Dariusz Wyrzykowski , Bogusław Pilarski , Damian Neubauer , Elżbieta Kamysz , Aleksandra Tesmar , Lech Chmurzyński
AbstractIsothermal titration calorimetry (ITC) and potentiometric titration (PT) methods were used to study theinteractions of copper(II) ions with GAG, GDG, GKG, and GHG peptides. The calorimetric measurementswere run in a MES buffer with the pH value of 6.0 at 298.15 K. Based on the results of PT data supportedby theoretical calculations, the dissociation constants were calculated for the investigated peptides. Thequantification of the proton competition with the metal for the peptide and incorporation it into the ITCdata analysis enabled to obtain the pH-independent parameters, namely the binding constants (K) andthe free energy of binding (DG). Furthermore, the relationship between the proposed coordination modesof the considered peptides and the thermodynamic parameters (K,DGandDH) has been discussed.
|Journal series||Journal of Chemical Thermodynamics, ISSN 0021-9614, (A 30 pkt)|
|Publication size in sheets||0.5|
|ASJC Classification||; ;|
|Score||= 30.0, 24-07-2019, ArticleFromJournal|
|Publication indicators||= 0; : 2017 = 1.111; : 2017 = 2.631 (2) - 2017=2.531 (5)|
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