Copper(II) coordination properties of GxG peptides: key role of sidechains of central residues on coordination of formed systems; combined potentiometric and ITC studies

Joanna Makowska , Dariusz Wyrzykowski , Bogusław Pilarski , Damian Neubauer , Elżbieta Kamysz , Aleksandra Tesmar , Lech Chmurzyński

Abstract

Isothermal titration calorimetry (ITC) and potentiometric titration (PT) methods were used to study theinteractions of copper(II) ions with GAG, GDG, GKG, and GHG peptides. The calorimetric measurementswere run in a MES buffer with the pH value of 6.0 at 298.15 K. Based on the results of PT data supportedby theoretical calculations, the dissociation constants were calculated for the investigated peptides. Thequantification of the proton competition with the metal for the peptide and incorporation it into the ITCdata analysis enabled to obtain the pH-independent parameters, namely the binding constants (K) andthe free energy of binding (DG). Furthermore, the relationship between the proposed coordination modesof the considered peptides and the thermodynamic parameters (K,DGandDH) has been discussed.
Author Joanna Makowska (FCh / DGICh / PBChN)
Joanna Makowska,,
- Pracownia Biologicznej Chemii Nieorganicznej
, Dariusz Wyrzykowski (FCh / DGICh / PBChN)
Dariusz Wyrzykowski,,
- Pracownia Biologicznej Chemii Nieorganicznej
, Bogusław Pilarski
Bogusław Pilarski,,
-
, Damian Neubauer
Damian Neubauer,,
-
, Elżbieta Kamysz (FCh / DMBt / LChBM)
Elżbieta Kamysz,,
- Laboratory of Chemistry of Biological Macromolecules
, Aleksandra Tesmar (FCh / DGICh / LPCC)
Aleksandra Tesmar,,
- Laboratory of Physicochemistry of Coordination Complexes
, Lech Chmurzyński (FCh / DGICh / LPCC)
Lech Chmurzyński,,
- Laboratory of Physicochemistry of Coordination Complexes
Journal seriesJournal of Chemical Thermodynamics, ISSN 0021-9614, (A 30 pkt)
Issue year2019
Vol128
Pages336-343
Publication size in sheets0.5
ASJC Classification1606 Physical and Theoretical Chemistry; 2500 General Materials Science; 3107 Atomic and Molecular Physics, and Optics
DOIDOI:10.1016/j.jct.2018.08.040
URL https://doi.org/10.1016/j.jct.2018.08.040
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 24-07-2019, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.111; WoS Impact Factor: 2017 = 2.631 (2) - 2017=2.531 (5)
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