The microbiome profiling of fungivorous black tinder fungus beetle Bolitophagus reticulatus reveals the insight into bacterial communities associated with larvae and adults

Agnieszka Kaczmarczyk-Ziemba , Grzegorz K. Wagner , Krzysztof Grzywnowicz , Marek Kucharczyk , Sylwia Zielińska

Abstract

Saproxylic beetles play a crucial role in key processes occurring in forest ecosystems, and together with fungi contribute to the decomposition and mineralization of wood. Among this group are mycetophilic beetles which associate with wood-decaying fungi and use the fruiting body for nourishment and development. Therefore, their feeding strategy (especially in the case of fungivorous species) requires special digestive capabilities to take advantage of the nutritional value of fungal tissue. Although polypore-beetle associations have been investigated in numerous studies, detailed studies focusing on the microbiome associated with species feeding on fruiting bodies of polypores remain limited. Here we investigated the bacterial communities associated with larvae and adults ofBolitophagus reticulatuscollected fromFomes fomentariusgrowing on two different host tree: beech (Fagussp.) and birch (Betulasp.), respectively. Among 24 identified bacterial phyla, three were the most relatively abundant (Proteobacteria, Actinobacteria and Bacteroidetes). Moreover, we tried to find unique patterns of bacteria abundances which could be correlated with the long-term field observation showing that the fruiting bodies ofF. fomentarius,growing on birch are more inhabited by beetles than fruiting bodies of the same fungus species growing on beech. Biochemical analyses showed that the level of protease inhibitors and secondary metabolites inF. fomentariusis higher in healthy fruiting bodies than in the inhabited ones. However, tested microbiome samples primarily clustered by developmental stage ofB. reticulatusand host tree did not appear to impact the taxonomic distribution of the communities. This observation was supported by statistical analyses.
Author Agnieszka Kaczmarczyk-Ziemba (FB / DGB)
Agnieszka Kaczmarczyk-Ziemba,,
- Katedra Genetyki i Biosystematyki
, Grzegorz K. Wagner
Grzegorz K. Wagner,,
-
, Krzysztof Grzywnowicz
Krzysztof Grzywnowicz,,
-
, Marek Kucharczyk
Marek Kucharczyk,,
-
, Sylwia Zielińska (FB / DMGB)
Sylwia Zielińska,,
- Department of Molecular Genetics of Bacteria
Journal seriesPeerJ, ISSN 2167-8359, (N/A 100 pkt)
Issue year2019
Vol7
Pages1-21
Publication size in sheets1
Keywords in EnglishSaproxylic species, bacterial community structure, 16S rRNA gene, next generation sequencing, protease inhibitors, secondary metabolites profiles, Bolitophagus reticulatus, Fomes fomentarius
ASJC Classification2700 General Medicine; 1100 General Agricultural and Biological Sciences; 1300 General Biochemistry, Genetics and Molecular Biology; 2800 General Neuroscience
DOIDOI:10.7717/peerj.6852
URL https://peerj.com/articles/6852/
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 29-11-2019, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.865; WoS Impact Factor: 2018 = 2.353 (2) - 2018=2.7 (5)
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