Development and application of novelty pretreatment method for the concurrent quantitation of eleven water-soluble B vitamins in ultrafiltrates after renal replacement therapy

Dorota Wirkus , Aleksandra Jakubus , Radosław Owczuk , Piotr Stepnowski , Monika Paszkiewicz


Continous renal replacement therapy (CRRT) is particularly recommended for septic shock patients in intensive care units. The CRRT technique used most frequently is high volume continuous veno-venous haemofiltration. It provides a high rate of clearance of uremic toxins and inflammatory cytokines. However, it should also be taken into account that substances important for homeostasis may be concurrently unintentionally removed. Accordingly, water-soluble vitamins can be removed during continuous renal replacement therapy, and the estimate of the loss is critical to ensure appropriate supplementation. The aim of this work was to develop a simple methodology for a purification step prior to the LC–MS/MS determination of water-soluble vitamins in ultrafiltrate samples. For this purpose, two types of resin and a mix of resins were used as sorbents for the purification step. Moreover, parameters such as the amount of resin and the extraction time were optimized. The LC–MS/MS method was developed and validated for final determination of 11 vitamins. The results demonstrated the high purification capability of DEAE Sephadex resin with recoveries between 65 and 101% for water-soluble vitamins from ultrafiltrate samples. An optimized method was applied to assess the loss of B-group vitamins in patients after 24 h of renal replacement therapy. The loss of vitamins B2, B6 pyridoxamine, B6 pyridoxal, B7, B1, and B5 in ultrafiltrates was similar in all patients. In the native ultrafiltrates, vitamins B6 pyridoxine, B9 and B12 were not detected.
Author Dorota Wirkus PChZŚ
Dorota Wirkus ,,
- Laboratory of Chemical Environmental Risks
, Aleksandra Jakubus PChZŚ
Aleksandra Jakubus,,
- Laboratory of Chemical Environmental Risks
, Radosław Owczuk
Radosław Owczuk,,
, Piotr Stepnowski PChZŚ
Piotr Stepnowski,,
- Laboratory of Chemical Environmental Risks
, Monika Paszkiewicz PAiMŚ
Monika Paszkiewicz,,
- Pracownia Analityki i Monitoringu Środowiska
Journal seriesJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, ISSN 1570-0232
Issue year2017
Publication size in sheets0.5
Keywords in Englishwater-soluble vitamins, ion exchange resins, LC-MS, high volume continuous veno-venou, haemofiltration, ultrafiltrates
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.603 (2) - 2016=2.711 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.