Development and application of novelty pretreatment method for the concurrent quantitation of eleven water-soluble B vitamins in ultrafiltrates after renal replacement therapy

Dorota Wirkus , Aleksandra Jakubus , Radosław Owczuk , Piotr Stepnowski , Monika Paszkiewicz


Continous renal replacement therapy (CRRT) is particularly recommended for septic shock patients in intensive care units. The CRRT technique used most frequently is high volume continuous veno-venous haemofiltration. It provides a high rate of clearance of uremic toxins and inflammatory cytokines. However, it should also be taken into account that substances important for homeostasis may be concurrently unintentionally removed. Accordingly, water-soluble vitamins can be removed during continuous renal replacement therapy, and the estimate of the loss is critical to ensure appropriate supplementation. The aim of this work was to develop a simple methodology for a purification step prior to the LC–MS/MS determination of water-soluble vitamins in ultrafiltrate samples. For this purpose, two types of resin and a mix of resins were used as sorbents for the purification step. Moreover, parameters such as the amount of resin and the extraction time were optimized. The LC–MS/MS method was developed and validated for final determination of 11 vitamins. The results demonstrated the high purification capability of DEAE Sephadex resin with recoveries between 65 and 101% for water-soluble vitamins from ultrafiltrate samples. An optimized method was applied to assess the loss of B-group vitamins in patients after 24 h of renal replacement therapy. The loss of vitamins B2, B6 pyridoxamine, B6 pyridoxal, B7, B1, and B5 in ultrafiltrates was similar in all patients. In the native ultrafiltrates, vitamins B6 pyridoxine, B9 and B12 were not detected.
Author Dorota Wirkus (FCh / DEA / LChER)
Dorota Wirkus ,,
- Laboratory of Chemical Environmental Risks
, Aleksandra Jakubus (FCh / DEA / LChER)
Aleksandra Jakubus,,
- Laboratory of Chemical Environmental Risks
, Radosław Owczuk - [Gdanski Uniwersytet Medyczny]
Radosław Owczuk,,
- Gdanski Uniwersytet Medyczny
, Piotr Stepnowski (FCh / DEA / LChER)
Piotr Stepnowski,,
- Laboratory of Chemical Environmental Risks
, Monika Paszkiewicz (FCh / DEA / PAiMŚ)
Monika Paszkiewicz,,
- Pracownia Analityki i Monitoringu Środowiska
Journal seriesJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, ISSN 1570-0232, (A 30 pkt)
Issue year2017
Publication size in sheets0.5
Keywords in Englishwater-soluble vitamins, ion exchange resins, LC-MS, high volume continuous veno-venou, haemofiltration, ultrafiltrates
ASJC Classification2700 General Medicine; 1303 Biochemistry; 1307 Cell Biology; 1308 Clinical Biochemistry; 1602 Analytical Chemistry
Languageen angielski
Not used for evaluationyes
Score (nominal)0
Publication indicators Scopus Citations = 5; WoS Citations = 3; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.989; WoS Impact Factor: 2017 = 2.441 (2) - 2017=2.526 (5)
Citation count*4 (2020-05-06)
Additional fields
UwagiArtykuł został zgłoszony do parametryzacji za lata 2013-2016
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