Characterization of Dickeya and Pectobacterium strains obtained from diseased potato plants in different climatic conditions of Norway and Poland

Merete Wiken Dees , Renata Lebecka , Juliana Irina Spies Perminow , Robert Czajkowski , Anna Grupa , Agata Motyka , Sabina Żołędowska , Jadwiga Śliwka , Ewa Łojkowska , May Bente Brurberg

Abstract

Soft rot and blackleg of potato caused by pectinolytic bacteria lead to severe economic losses in potato production worldwide. To investigate the species composition of bacteria causing soft rot and black leg of potato in Norway and Poland, bacteria were isolated from potato tubers and stems. Forty-one Norwegian strains and 42 Polish strains that formed cavities on pectate medium were selected for potato tuber maceration assays and sequencing of three housekeeping genes (dnaX, icdA and mdh) for species identification and phylogenetic analysis. The distribution of the species causing soft rot and blackleg in Norway and Poland differed: we have demonstrated that mainly P. atrosepticum and P. c. subsp. carotovorum are the causal agents of soft rot and blackleg of potatoes in Norway, while P. wasabiae was identified as one of the most important soft rot pathogens in Poland. In contrast to the other European countries, D. solani seem not to be a major pathogen of potato in Norway and Poland. The Norwegian and Polish P. c. subsp. carotovorum and P. wasabiae strains did not cluster with type strains of the respective species in the phylogenetic analysis, which underlines the taxonomic complexity of the genus Pectobacterium. No correlation between the country of origin and clustering of the strains was observed. All strains tested in this study were able to macerate potato tissue. The ability to macerate potato tissue was significantly greater for the P. c. subsp. carotovorum and Dickeya spp., compared to P. atrosepticum and P. wasabiae.
Author Merete Wiken Dees - [The Norwegian Institute of Bioeconomy Research]
Merete Wiken Dees,,
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, Renata Lebecka - [Instytut Hodowli i Aklimatyzacji Roślin]
Renata Lebecka,,
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, Juliana Irina Spies Perminow - [The Norwegian Institute of Bioeconomy Research]
Juliana Irina Spies Perminow,,
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, Robert Czajkowski (IFB / IB / DBiotech)
Robert Czajkowski,,
- Department of Biotechnology
, Anna Grupa - [Instytut Hodowli i Aklimatyzacji Roślin]
Anna Grupa,,
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, Agata Motyka (IFB)
Agata Motyka,,
- Intercollegiate Faculty of Biotechnology UG
, Sabina Żołędowska (IFB)
Sabina Żołędowska,,
- Intercollegiate Faculty of Biotechnology UG
, Jadwiga Śliwka - [Instytut Hodowli i Aklimatyzacji Roślin]
Jadwiga Śliwka,,
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, Ewa Łojkowska (IFB / IB / DBiotech)
Ewa Łojkowska,,
- Department of Biotechnology
, May Bente Brurberg - [The Norwegian Institute of Bioeconomy Research]
May Bente Brurberg,,
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Other language title versions
Journal seriesEuropean Journal of Plant Pathology, ISSN 0929-1873, (A 30 pkt)
Issue year2017
Vol148
No4
Pages839-851
Publication size in sheets0.6
Keywords in Englishblack leg, pectinolyticbacteria, phylogeny, soft rot, Solanum tuberosum
ASJC Classification1108 Horticulture; 1110 Plant Science; 1102 Agronomy and Crop Science
DOIDOI:10.1007/s10658-016-1140-2
URL http://link.springer.com/content/pdf/10.1007%2Fs10658-016-1140-2.pdf
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)30
Score sourcejournalList
ScoreMinisterial score = 30.0, 08-11-2019, ArticleFromJournal
Publication indicators WoS Citations = 15; Scopus Citations = 16; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.046; WoS Impact Factor: 2017 = 1.466 (2) - 2017=1.728 (5)
Citation count*22 (2019-11-07)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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