Alanine scanning studies of the antimicrobial peptide aurein 1.2

Dorian Migoń , Maciej Jaśkiewicz , Damian Neubauer , Marta Bauer , Emilia Sikorska , Elżbieta Kamysz , Wojciech Kamysz

Abstract

Antimicrobial peptides (AMPs) are compounds widely distributed in nature that display activity against a broad spectrum of pathogens. Amphibian skin, as an organ rich in pharmacologically active peptides, appears to be an interesting source of novel AMPs. Aurein 1.2 (GLFDIIKKIAESF-NH2) is a short 13-residue antimicrobial peptide primarily isolated from the skin secretions of Australian bell frogs. In this study, the alanine scan of aurein 1.2 was performed to investigate the effect of each amino acid residue on its biological and physico-chemical properties. The biological studies included determination of minimum inhibitory concentration, activity against biofilm, and inhibitory effect on its formation. Moreover, the hemolytic activity as well as serum stability was determined. The hydrophobicity of peptides and their self-assembly were investigated using reversed-phase chromatography. In addition, their helicitywas calculated from circular dichroismspectra. The results not only provided information on structure-activity relationship of aurein 1.2 but also gave insights into design of novel analogs of AMPs in the future.
Publication typeIn press (online first, early view)
Author Dorian Migoń
Dorian Migoń,,
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, Maciej Jaśkiewicz
Maciej Jaśkiewicz,,
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, Damian Neubauer
Damian Neubauer,,
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, Marta Bauer
Marta Bauer,,
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, Emilia Sikorska (FCh / DOCh / PBSB)
Emilia Sikorska,,
- Pracownia Badań Strukturalnych Biopolimerów
, Elżbieta Kamysz (FCh / DMBt / LChBM)
Elżbieta Kamysz,,
- Laboratory of Chemistry of Biological Macromolecules
, Wojciech Kamysz
Wojciech Kamysz,,
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Journal seriesProbiotics and Antimicrobial Proteins, ISSN 1867-1306, (A 20 pkt)
Issue year2018
Noonline first
Pages1-13
Publication size in sheets0.6
Keywords in EnglishAurein 1.2, antimicrobial peptides, structure-activity relationship, antimicrobial agents, peptide drugs, antibiotics
ASJC Classification1312 Molecular Biology; 1313 Molecular Medicine; 2404 Microbiology
DOIDOI:10.1007/s12602-018-9501-0
URL https://doi.org/10.1007/s12602-018-9501-0
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)20
ScoreMinisterial score = 20.0, 03-04-2019, ArticleFromJournal
Ministerial score (2013-2016) = 20.0, 03-04-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.564; WoS Impact Factor: 2017 = 2.345 (2) - 2017=2.091 (5)
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