Isolation and characterization of plant growth-promoting endophytic fungi from the roots of Dendrobium moniliforme

Sujit Shah , Roshani Shrestha , Sabitri Maharjan , Marc-Andre Selosse , Bijaya Pant

Abstract

The present study aims to identify the diverse endophytic fungi residing in the roots of Dendrobium moniliforme and their role in plant growth and development. Nine endophytic fungi were isolated from the root sections and characterized by molecular technique. Quantification of the indole acetic acid (IAA) compound by these endophytes was done. Further, Chemical profiling of R11 and R13 fungi was done by Gas Chromatography-Mass Spectroscopy (GC-MS). Asymbiotic seed derived protocorms of Rhynchostylis retusa was used for the plant growth assay to investigate the growth promoting activities of the fungal elicitor prepared from the isolated fungi from D. moniliforme. Among the isolated fungi, the relative dominant fungus was Fusarium sp. The R13 and R6 fungi were identified only at the genus level which concludes the fungi are of new species or strain. The indole acetic acid production was relatively higher in R10. Bioactive compound diversity was observed in the organic extract of R11 and R6. The presence of phenolic compound and essential oil suggest their contribution for the antimicrobial and antioxidant properties to their host plant, D. moniliforme. The plant growth assay result concluded, the fungal elicitor prepared from R10, Colletotrichum alatae was the best among all other for the plant growth activities.
Author Sujit Shah
Sujit Shah,,
-
, Roshani Shrestha
Roshani Shrestha,,
-
, Sabitri Maharjan
Sabitri Maharjan,,
-
, Marc-Andre Selosse (FB / DPTNC)
Marc-Andre Selosse,,
- Department of Plant Taxonomy and Nature Conservation
, Bijaya Pant
Bijaya Pant,,
-
Journal seriesPlants, ISSN , e-ISSN 2223-7747, (0 pkt)
Issue year2019
Vol8
No1
Pages1-11
Publication size in sheets0.5
Keywords in Englishorchid, Dendrobium moniliforme, endophytes, elicitor, indole acetic acid
DOIDOI:10.3390/plants8010005
URL https://doi.org/10.3390/plants8010005
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)5
ScoreMinisterial score = 0.0, 05-02-2019, ArticleFromJournal
Ministerial score (2013-2016) = 5.0, 05-02-2019, ArticleFromJournal - czasopismo zagraniczne spoza list
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