Copper(II) complexation by fragment of central part of FBP28 protein from Mus musculus

Joanna Makowska , Krzysztof Żamojć , Dariusz Wyrzykowski , Wiesław Wiczk , Lech Chmurzyński

Abstract

Steady-state and time-resolved fluorescence spectroscopy, UV spectrophotometry and isothermal titration calorimetry techniques were used to study the coordinating properties of the 17aa peptide fragment (D17) derived from the central part of the mouse formin binding protein (FBP28 with the PDB code: 1E0L) towards Cu2+ ions. All the measurements were run in the 2-(N-morpholino)ethanesulfonic acid buffer (20 mM, pH 6.0). Under experimental conditions the formation of the 1:1 complex of Cu2+ ions with D17 is an entropy-driven process. Cu2+ ions cause the static fluorescence quenching of the peptide studied through the formation of a nonfluorescent complex. Furthermore, the thermal stability of D17 was discussed based on the results obtained from differential scanning fluorimetry (nanoDSF) data.
Author Joanna Makowska (FCh / DGICh / PBChN)
Joanna Makowska,,
- Pracownia Biologicznej Chemii Nieorganicznej
, Krzysztof Żamojć (FCh / DGICh / LPCC)
Krzysztof Żamojć,,
- Laboratory of Physicochemistry of Coordination Complexes
, Dariusz Wyrzykowski (FCh / DGICh / PBChN)
Dariusz Wyrzykowski,,
- Pracownia Biologicznej Chemii Nieorganicznej
, Wiesław Wiczk (FCh / DBCh / LP)
Wiesław Wiczk,,
- Laboratory of Photobiophysics
, Lech Chmurzyński (FCh / DGICh / LPCC)
Lech Chmurzyński,,
- Laboratory of Physicochemistry of Coordination Complexes
Journal seriesBiophysical Chemistry, ISSN 0301-4622, (A 20 pkt)
Issue year2018
Vol241
Pages55-60
Publication size in sheets0.5
Keywords in EnglishMetal-peptide binding, fluorescence quenching, isothermal titration calorimetry, thermodynamic parameters, UV spectroscopy
ASJC Classification1605 Organic Chemistry; 1303 Biochemistry; 1304 Biophysics
DOIDOI:10.1016/j.bpc.2018.08.002
URL https://doi.org/10.1016/j.bpc.2018.08.002
Languageen angielski
Score (nominal)20
ScoreMinisterial score = 20.0, 30-09-2019, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.78; WoS Impact Factor: 2017 = 1.87 (2) - 2017=1.963 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back