The uniform structure of O-polysaccharides isolated from Dickeya solani strains of different origin

Karolina Ossowska , Małgorzata Czerwicka , Wojciech Śledź , Sabina Żołędowska , Agata Motyka , Małgorzata Golanowska , Guy Condemine , Ewa Łojkowska , Zbigniew Kaczyński

Abstract

O-polysaccharides were isolated from lipopolysaccharides obtained from four different strains of plant pathogenic bacteria belonging to the species Dickeya solani: two of them were isolated in Poland (IFB0099 and IFB0158), the third in Germany (IFB0223) and the last one, D. solani Type Strain IPO2222, originated from the Netherlands. In addition, the O-polysaccharide of a closely related species D. dadantii strain 3937 was isolated. The purified polysaccharides of the five strains were analyzed using NMR spectroscopy and chemical methods. Sugar and methylation analyses, including absolute configuration assignment, together with NMR data revealed that all O-polysaccharides tested are homopolymers of 6-deoxy-d-altrose (d-6dAlt) the following structure: →2)-β-d-6dAltp-(1→
Author Karolina Ossowska PBS
Karolina Ossowska,,
- Laboratory of Structural Biochemistry
, Małgorzata Czerwicka PBS
Małgorzata Czerwicka,,
- Laboratory of Structural Biochemistry
, Wojciech Śledź KB
Wojciech Śledź,,
- Department of Biotechnology
, Sabina Żołędowska MWB UG i GUMed
Sabina Żołędowska,,
- Intercollegiate Faculty of Biotechnology UG
, Agata Motyka MWB UG i GUMed
Agata Motyka,,
- Intercollegiate Faculty of Biotechnology UG
, Małgorzata Golanowska KB
Małgorzata Golanowska,,
- Department of Biotechnology
, Guy Condemine
Guy Condemine,,
-
, Ewa Łojkowska KB
Ewa Łojkowska,,
- Department of Biotechnology
, Zbigniew Kaczyński PBS
Zbigniew Kaczyński,,
- Laboratory of Structural Biochemistry
Other language title versions
Journal seriesCarbohydrate Research, ISSN 0008-6215
Issue year2017
Vol445
Pages40-43
Publication size in sheets0.5
Keywords in EnglishDickeya solani, Dickeya dadantii, O-polysaccharide, structure, NMR, 6-Deoxy-D-altrose
DOIDOI:10.1016/j.carres.2017.04.001
URL http://doi.org/10.1016/j.carres.2017.04.001
Languageen angielski
Score (nominal)25
ScoreMinisterial score = 25.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.096 (2) - 2016=1.963 (5)
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