The uniform structure of O-polysaccharides isolated from Dickeya solani strains of different origin

Karolina Ossowska , Małgorzata Czerwicka , Wojciech Śledź , Sabina Żołędowska , Agata Motyka , Małgorzata Golanowska , Guy Condemine , Ewa Łojkowska , Zbigniew Kaczyński


O-polysaccharides were isolated from lipopolysaccharides obtained from four different strains of plant pathogenic bacteria belonging to the species Dickeya solani: two of them were isolated in Poland (IFB0099 and IFB0158), the third in Germany (IFB0223) and the last one, D. solani Type Strain IPO2222, originated from the Netherlands. In addition, the O-polysaccharide of a closely related species D. dadantii strain 3937 was isolated. The purified polysaccharides of the five strains were analyzed using NMR spectroscopy and chemical methods. Sugar and methylation analyses, including absolute configuration assignment, together with NMR data revealed that all O-polysaccharides tested are homopolymers of 6-deoxy-d-altrose (d-6dAlt) the following structure: →2)-β-d-6dAltp-(1→
Author Karolina Ossowska (FCh/DBCh/LSB)
Karolina Ossowska,,
- Laboratory of Structural Biochemistry
, Małgorzata Czerwicka (FCh/DBCh/LSB)
Małgorzata Czerwicka,,
- Laboratory of Structural Biochemistry
, Wojciech Śledź (IFB/IB/DBiotech)
Wojciech Śledź,,
- Department of Biotechnology
, Sabina Żołędowska (IFB)
Sabina Żołędowska,,
- Intercollegiate Faculty of Biotechnology UG
, Agata Motyka (IFB)
Agata Motyka,,
- Intercollegiate Faculty of Biotechnology UG
, Małgorzata Golanowska (IFB/IB/DBiotech)
Małgorzata Golanowska,,
- Department of Biotechnology
, Guy Condemine - [Microbiologie, Adaptation et Pathogénie]
Guy Condemine,,
, Ewa Łojkowska (IFB/IB/DBiotech)
Ewa Łojkowska,,
- Department of Biotechnology
, Zbigniew Kaczyński (FCh/DBCh/LSB)
Zbigniew Kaczyński,,
- Laboratory of Structural Biochemistry
Other language title versions
Journal seriesCarbohydrate Research, ISSN 0008-6215, (A 25 pkt)
Issue year2017
Publication size in sheets0.50
Keywords in EnglishDickeya solani, Dickeya dadantii, O-polysaccharide, structure, NMR, 6-Deoxy-D-altrose
ASJC Classification1605 Organic Chemistry; 1303 Biochemistry; 2700 General Medicine; 1602 Analytical Chemistry
Languageen angielski
Score (nominal)25
Score sourcejournalList
ScoreMinisterial score = 25.0, 07-08-2020, ArticleFromJournal
Publication indicators WoS Citations = 1.000; Scopus Citations = 2.000; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.754; WoS Impact Factor: 2017 = 2.074 (2) - 2017=1.921 (5)
Citation count*3 (2020-08-10)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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