Mature Luffa Leaves (Luffa cylindrica L.) as a tool for gene expression analysis by agroinfiltration

Kamila Błażejewska , Małgorzata Kapusta , Elżbieta Zielińska , Zbigniew Tukaj , Izabela Chincinska

Abstract

We exploited the potential of cucurbits for ectopic gene expression. Agroinfiltration is a simple and commonly used method to obtain transient expression of foreign genes in plants. In contrast to in vitro transformation techniques, agroinfiltration can be used for genetic modification of mature plant tissues. Although the cucurbits are commonly used as model plants for molecular biology and biotechnology studies, to date there are no literature sources on the possibility of transient gene expression in mature cucurbit tissues. Our research has shown that mature leaves of Luffa cylindrica L. (luffa), in contrast to other cucurbit species, can be successfully transiently transformed with Agrobacterium tumefaciens. We efficiently transformed luffa leaves with a reporter gene encoding β-glucuronidase (GUS). The GUS activity in transiently transformed leaf tissues was detected within 24 h after the infiltration with bacteria. Additionally, we have shown that the activity of a transiently expressed the GUS gene can be monitored directly in the EDTA-exudates collected from the cut petioles of the agroinfiltrated leaves. The results suggest that luffa leaves can be useful as a plant expression system for studies of physiological and biochemical processes in cucurbits.
Author Kamila Błażejewska KFiBR
Kamila Błażejewska,,
- Department of Plant Physiology and Biotechnology
, Małgorzata Kapusta KCiER
Małgorzata Kapusta,,
- Department of Plant Cytology and Embryology
, Elżbieta Zielińska KFiBR
Elżbieta Zielińska,,
- Department of Plant Physiology and Biotechnology
, Zbigniew Tukaj KFiBR
Zbigniew Tukaj,,
- Department of Plant Physiology and Biotechnology
, Izabela Chincinska KFiBR
Izabela Chincinska,,
- Department of Plant Physiology and Biotechnology
Other language title versions
Journal seriesFrontiers in Plant Science, ISSN 1664-462X
Issue year2017
Vol8
Pages1-10
Publication size in sheets0.5
Keywords in Englishcucurbits, agroinfiltration, Luffa, phloem exudate, plant overexpression system, foliar application of tracers
DOIDOI:10.3389/fpls.2017.00228
URL http://journal.frontiersin.org/article/10.3389/fpls.2017.00228/full
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 40.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.291 (2) - 2016=4.672 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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