Mature Luffa Leaves (Luffa cylindrica L.) as a tool for gene expression analysis by agroinfiltration
Kamila Błażejewska , Małgorzata Kapusta , Elżbieta Zielińska , Zbigniew Tukaj , Izabela Chincinska
AbstractWe exploited the potential of cucurbits for ectopic gene expression. Agroinfiltration is a simple and commonly used method to obtain transient expression of foreign genes in plants. In contrast to in vitro transformation techniques, agroinfiltration can be used for genetic modification of mature plant tissues. Although the cucurbits are commonly used as model plants for molecular biology and biotechnology studies, to date there are no literature sources on the possibility of transient gene expression in mature cucurbit tissues. Our research has shown that mature leaves of Luffa cylindrica L. (luffa), in contrast to other cucurbit species, can be successfully transiently transformed with Agrobacterium tumefaciens. We efficiently transformed luffa leaves with a reporter gene encoding β-glucuronidase (GUS). The GUS activity in transiently transformed leaf tissues was detected within 24 h after the infiltration with bacteria. Additionally, we have shown that the activity of a transiently expressed the GUS gene can be monitored directly in the EDTA-exudates collected from the cut petioles of the agroinfiltrated leaves. The results suggest that luffa leaves can be useful as a plant expression system for studies of physiological and biochemical processes in cucurbits.
|Other language title versions|
|Journal series||Frontiers in Plant Science, ISSN 1664-462X, (A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||cucurbits, agroinfiltration, Luffa, phloem exudate, plant overexpression system, foliar application of tracers|
|License||Journal (articles only); published final; ; with publication|
|Score||= 40.0, 29-05-2020, ArticleFromJournal|
|Publication indicators||= 0; = 2; : 2017 = 1.232; : 2017 = 3.678 (2) - 2017=4.354 (5)|
|Citation count*||3 (2020-05-16)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.