Phylogenetic positions of seven poorly known species of Ferula (Apiaceae) with remarks on the phylogenetic utility of the plastid trnH-psbA, trnS-trnG, and atpB-rbcL intergenic spacers

Marcin Piwczyński , Dominika Wyborska , Joanna Gołębiewska , Radosław Puchałka

Abstract

Ferula L. is one of the most species-rich and taxonomically difficult genera of Apiaceae. In this study, we obtained nrDNA ITS sequences of seven poorly known species of Ferula (Ferula anatolica, Ferula sp. (tentatively identified as F. candelabrum by collectors), F. drudeana, F. huber-morathii, F. marmarica, F. talassica, and F. tunetana) and explored their phylogenetic positions using 148 ITS sequences of the subtribe Ferulinae from GenBank. Five of these newly sequenced species fall into three groups, corresponding to clades recognized in earlier molecular studies. Ferula sp. are added to clade, which is mostly composed of Central Asian species. This placement showed that identification as F. candelabrum was erroneous. The second clade, which is mostly composed of Mediterranean taxa, includes two species from North Africa: F. marmarica and F. tunetana. Despite the well-supported monophyly of this clade, the relationships inside this group need to be revised, as broadly distributed F. communis is paraphyletic with respect to other species. Ferula drudeana and F. huber-morathii, two narrow endemics from Turkey, are placed in the Central Asian clade. Two species, F. anatolica and F. talassica, do not fall into any of the recognized clades. In addition, we examined the sequence variation of three potentially highly variable pDNA regions, the trnH-psbA, trnS-trnG, and atpB-rbcL intergenic spacers, for a subset of 18 specimens. The resulting pDNA and ITS based phylogenetic trees were incongruent, as supported by significant ILD tests. The cause of this incongruence can be manifold, including hybridization, a lack of a phylogenetic signal, and homoplastic substitutions. Our analyses suggest that only trnS-trnG can be added to the list of pDNA markers used for phylogenetic studies of Ferula, as it has the highest number of parsimony informative characters and is easy to amplify from degraded material.
Publication typeIn press (online first, early view)
Author Marcin Piwczyński
Marcin Piwczyński,,
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, Dominika Wyborska
Dominika Wyborska,,
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, Joanna Gołębiewska KTRiOP
Joanna Gołębiewska,,
- Department of Plant Taxonomy and Nature Conservation
, Radosław Puchałka
Radosław Puchałka,,
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Journal seriesSystematics and Biodiversity, ISSN 1477-2000
Issue year2018
Pages1-13
Publication size in sheets0.6
Keywords in EnglishFerulinae, herbarium material, ILD test, nrDNA ITS, pDNA, umbellifers
DOIDOI:10.1080/14772000.2018.1442374
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 03-04-2018, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 03-04-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.127 (2) - 2016=2.141 (5)
Citation count*0
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