Optimization of LC method for the quantification of doxorubicin in plasma and urine samples in view of pharmacokinetic, biomedical and drug monitoring therapy studies
Olga Maliszewska , Alina Plenis , Ilona Olędzka , Piotr Kowalski , Natalia Miękus-Purwin , Ewa Bień , Małgorzata Anna Krawczyk , Elżbieta Adamkiewicz-Drożynska , Tomasz Bączek
AbstractA simple, rapid, reliable and sensitive method based on liquid chromatography with fluorescence detection (LC-FL) for the quantification of doxorubicin (DOX) in human plasma and urine samples was developed. The assay was carried out after the solid-phase extraction procedure (SPE) with hydrophilic-lipophilic balance (HLB) cartridges, and with daunorubicin hydrochloride (DAU) used as the internal standard. Chromatographic separation was performed on a Discovery HS C18 column in isocratic elution mode, and the detection of the analytes set at excitation and emission wavelengths of 487 and 555 nm, respectively. The developed LC-FL method has been validated for accuracy, precision, selectivity, linearity, recovery and stability. The limits of detection and quantification for DOX were 0.5 and 1 ng/mL in both biological fluids, respectively. Linearity was confirmed in the range of 1–1000 ng/mL and 0.001–25 μg/mL in plasma and urine samples, respectively, with a correlation coefficient greater than 0.9994. The proposed LC-FL method is selective, precise and accurate, and has been successfully applied for drug monitoring in pediatric cancer patients treated with DOX as a component of OEPA (Oncovin (Vincristine)-Etoposide-Prednisone-Adriamycin) and IOA (Ifosfamide-Oncovin-Adriamycin) chemotherapeutic schemes. Moreover, real exposure of hospital personnel to the anthracycline drugs in plasma and urine was evaluated in clinical practice.
|Journal series||Journal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, (A 35 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||doxorubicin, plasma, urine, liquid chromatography, fluorescence detection, drug monitoring|
|Score|| = 35.0, ArticleFromJournal|
= 35.0, ArticleFromJournal
|Publication indicators||: 2017 = 2.831 (2) - 2017=2.863 (5)|
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