Optimization of LC method for the quantification of doxorubicin in plasma and urine samples in view of pharmacokinetic, biomedical and drug monitoring therapy studies

Olga Maliszewska , Alina Plenis , Ilona Olędzka , Piotr Kowalski , Natalia Miękus-Purwin , Ewa Bień , Małgorzata Anna Krawczyk , Elżbieta Adamkiewicz-Drożynska , Tomasz Bączek

Abstract

A simple, rapid, reliable and sensitive method based on liquid chromatography with fluorescence detection (LC-FL) for the quantification of doxorubicin (DOX) in human plasma and urine samples was developed. The assay was carried out after the solid-phase extraction procedure (SPE) with hydrophilic-lipophilic balance (HLB) cartridges, and with daunorubicin hydrochloride (DAU) used as the internal standard. Chromatographic separation was performed on a Discovery HS C18 column in isocratic elution mode, and the detection of the analytes set at excitation and emission wavelengths of 487 and 555 nm, respectively. The developed LC-FL method has been validated for accuracy, precision, selectivity, linearity, recovery and stability. The limits of detection and quantification for DOX were 0.5 and 1 ng/mL in both biological fluids, respectively. Linearity was confirmed in the range of 1–1000 ng/mL and 0.001–25 μg/mL in plasma and urine samples, respectively, with a correlation coefficient greater than 0.9994. The proposed LC-FL method is selective, precise and accurate, and has been successfully applied for drug monitoring in pediatric cancer patients treated with DOX as a component of OEPA (Oncovin (Vincristine)-Etoposide-Prednisone-Adriamycin) and IOA (Ifosfamide-Oncovin-Adriamycin) chemotherapeutic schemes. Moreover, real exposure of hospital personnel to the anthracycline drugs in plasma and urine was evaluated in clinical practice.
Author Olga Maliszewska
Olga Maliszewska,,
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, Alina Plenis
Alina Plenis,,
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, Ilona Olędzka
Ilona Olędzka,,
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, Piotr Kowalski
Piotr Kowalski,,
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, Natalia Miękus-Purwin (FB / DAHP)
Natalia Miękus-Purwin,,
- Department of Animal and Human Physiology
, Ewa Bień
Ewa Bień,,
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, Małgorzata Anna Krawczyk
Małgorzata Anna Krawczyk,,
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, Elżbieta Adamkiewicz-Drożynska
Elżbieta Adamkiewicz-Drożynska,,
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, Tomasz Bączek
Tomasz Bączek,,
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Journal seriesJournal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, (A 35 pkt)
Issue year2018
Vol158
Pages376-385
Publication size in sheets0.5
Keywords in Englishdoxorubicin, plasma, urine, liquid chromatography, fluorescence detection, drug monitoring
DOIDOI:10.1016/j.jpba.2018.06.031
URL https://doi.org/10.1016/j.jpba.2018.06.031
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, 27-06-2018, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 27-06-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.255 (2) - 2016=2.953 (5)
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