The effect of one anastomosis gastric bypass on branched-chain fatty acid and branched-chain amino acid metabolism in subjects with morbid obesity
Alicja Pakiet , Maciej Wilczyński , Olga Rostkowska , Justyna Korczyńska , Patrycja Jabłońska , Łukasz Kaska , Monika Proczko-Stepaniak , Ewa Sobczak , Piotr Stepnowski , Faidon Magkos , Tomasz Śledziński , Adriana Mika
AbstractBackground Subjects with morbid obesity have low levels of serum branched-chain fatty acids (BCFAs), which correlate inversely with insulin resistance, hypertriglyceridemia, and inflammation. Recent evidence suggests BCFAs are produced duringbranched-chain amino acid (BCAA) catabolism in human adipose tissue. Elevated concentrations of BCAAs are associated with insulin resistance. Objectives In this single-center study, we evaluated the effect of one anastomosis gastric bypass (OAGB) on circulating BCFAand BCAA. Moreover, we determined the expression of genes involved in BCAA catabolism in adipose tissue of patients with obesity and lean controls. Methods Fasting levels of BCFAs and BCAAs were determined by gas and liquid chromatography, respectively, coupled with mass spectrometry, in 50 patients with morbid obesity before and 6–9 months after surgery, and in 32 lean controls. Visceral and subcutaneous adipose tissue (VAT and SAT, respectively) biopsies were collected at baseline to determine mRNA levels forenzymes involved in BCAA catabolism. Results Before surgery, patients with obesity had lower BCFAs and greater BCAAs than control subjects. OAGB increased BCFA and decreased BCAA levels. Insulin resistance (assessed by HOMA) correlated inversely with BCFAs and positively with BCAAs. Expression of genes involved in BCAA catabolism in VAT (but not SAT) was lower in patients with obesity than in lean controls.
|Journal series||Obesity Surgery, ISSN 0960-8923, (N/A 100 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||branched-chain amino acids, branched-chain fatty acids, bariatric surgery, morbid obesity, insulin resistance|
|ASJC Classification||; ;|
|License||Other; published final; ; with publication|
|Score||= 100.0, 21-02-2020, ArticleFromJournal|
|Publication indicators||= 1; : 2016 = 1.328; : 2018 = 3.603 (2) - 2018=3.824 (5)|
|Citation count*||1 (2020-02-09)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.