The effect of one anastomosis gastric bypass on branched-chain fatty acid and branched-chain amino acid metabolism in subjects with morbid obesity

Alicja Pakiet , Maciej Wilczyński , Olga Rostkowska , Justyna Korczyńska , Patrycja Jabłońska , Łukasz Kaska , Monika Proczko-Stepaniak , Ewa Sobczak , Piotr Stepnowski , Faidon Magkos , Tomasz Śledziński , Adriana Mika

Abstract

Background Subjects with morbid obesity have low levels of serum branched-chain fatty acids (BCFAs), which correlate inversely with insulin resistance, hypertriglyceridemia, and inflammation. Recent evidence suggests BCFAs are produced duringbranched-chain amino acid (BCAA) catabolism in human adipose tissue. Elevated concentrations of BCAAs are associated with insulin resistance. Objectives In this single-center study, we evaluated the effect of one anastomosis gastric bypass (OAGB) on circulating BCFAand BCAA. Moreover, we determined the expression of genes involved in BCAA catabolism in adipose tissue of patients with obesity and lean controls. Methods Fasting levels of BCFAs and BCAAs were determined by gas and liquid chromatography, respectively, coupled with mass spectrometry, in 50 patients with morbid obesity before and 6–9 months after surgery, and in 32 lean controls. Visceral and subcutaneous adipose tissue (VAT and SAT, respectively) biopsies were collected at baseline to determine mRNA levels forenzymes involved in BCAA catabolism. Results Before surgery, patients with obesity had lower BCFAs and greater BCAAs than control subjects. OAGB increased BCFA and decreased BCAA levels. Insulin resistance (assessed by HOMA) correlated inversely with BCFAs and positively with BCAAs. Expression of genes involved in BCAA catabolism in VAT (but not SAT) was lower in patients with obesity than in lean controls.
Author Alicja Pakiet (FCh / DEA / LChER)
Alicja Pakiet,,
- Laboratory of Chemical Environmental Risks
, Maciej Wilczyński - [Gdanski Uniwersytet Medyczny]
Maciej Wilczyński,,
-
- Gdanski Uniwersytet Medyczny
, Olga Rostkowska - [Gdanski Uniwersytet Medyczny]
Olga Rostkowska,,
-
- Gdanski Uniwersytet Medyczny
, Justyna Korczyńska - [Gdanski Uniwersytet Medyczny]
Justyna Korczyńska,,
-
- Gdanski Uniwersytet Medyczny
, Patrycja Jabłońska - [Gdanski Uniwersytet Medyczny]
Patrycja Jabłońska,,
-
- Gdanski Uniwersytet Medyczny
, Łukasz Kaska - [Gdanski Uniwersytet Medyczny]
Łukasz Kaska,,
-
- Gdanski Uniwersytet Medyczny
, Monika Proczko-Stepaniak - [Gdanski Uniwersytet Medyczny]
Monika Proczko-Stepaniak,,
-
- Gdanski Uniwersytet Medyczny
, Ewa Sobczak - [Gdanski Uniwersytet Medyczny]
Ewa Sobczak,,
-
- Gdanski Uniwersytet Medyczny
, Piotr Stepnowski (FCh / DEA / LChER)
Piotr Stepnowski,,
- Laboratory of Chemical Environmental Risks
, Faidon Magkos - [Københavns Universitet]
Faidon Magkos,,
-
-
et al.`
Journal seriesObesity Surgery, ISSN 0960-8923, (N/A 100 pkt)
Issue year2020
Vol30
No1
Pages304-312
Publication size in sheets0.5
Keywords in Englishbranched-chain amino acids, branched-chain fatty acids, bariatric surgery, morbid obesity, insulin resistance
ASJC Classification2712 Endocrinology, Diabetes and Metabolism; 2746 Surgery; 2916 Nutrition and Dietetics
DOIDOI:10.1007/s11695-019-04157-z
URL https://link.springer.com/content/pdf/10.1007%2Fs11695-019-04157-z.pdf
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 21-02-2020, ArticleFromJournal
Publication indicators Scopus Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.328; WoS Impact Factor: 2018 = 3.603 (2) - 2018=3.824 (5)
Citation count*1 (2020-02-09)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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