Asymbiotic germination, seedling development and plantlet propagation of Encyclia aff. oncidioides - an endangered orchid

Joanna Znaniecka , Aleksandra Królicka , Matylda Sidwa-Gorycka , Jan J. Rybczyński , Dariusz Lucjan Szlachetko , Ewa Łojkowska

Abstract

In order to estimate the best germination conditions of Encyclia aff. oncidioides seeds, five different media (Fast, Knudson C modified by Vajrabhaya, Murashige and Skoog, PB2 and modified Vacin and Went) with different concentrations of plant growth regulators such as benzyladenine (BA), naphthaleneacetic acid (NAA) and gibberellic acid (GA3) were tested. No beneficial effect was observed when BA and NAA were applied to the germination medium and GA3 inhibited germination. The effect of light, activated charcoal, coconut water and casein hydrolysate on seed germination was also studied. The growth rate of seedlings on three different media supplemented with activated charcoal and plant growth regulators was checked. The applied plant growth regulators had no beneficial effect on the further growth of seedlings. Fast and PB2 media with 0.2% activated charcoal proved to be the best for E. aff. oncidioides seed germination, seedling development and plantlet propagation.
Author Joanna Znaniecka (IFB / IB / DBiotech)
Joanna Znaniecka,,
- Department of Biotechnology
, Aleksandra Królicka (IFB / IB / DBiotech)
Aleksandra Królicka,,
- Department of Biotechnology
, Matylda Sidwa-Gorycka (IFB)
Matylda Sidwa-Gorycka,,
- Intercollegiate Faculty of Biotechnology UG
, Jan J. Rybczyński - [Polish Academy of Sciences]
Jan J. Rybczyński,,
-
- Polish Academy of Sciences
, Dariusz Lucjan Szlachetko (FB / DPTNC)
Dariusz Lucjan Szlachetko,,
- Department of Plant Taxonomy and Nature Conservation
, Ewa Łojkowska (IFB / IB / DBiotech)
Ewa Łojkowska,,
- Department of Biotechnology
Journal seriesActa Societatis Botanicorum Poloniae, ISSN 0001-6977, e-ISSN 2083-9480, (A 15 pkt)
Issue year2005
Vol74
No3
Pages193-198
Keywords in Englishsymbiotic germination, endangered plants, in vitro propagation, orchids
ASJC Classification1110 Plant Science
Languageen angielski
Score (nominal)15
Score sourcejournalList
Publication indicators Scopus Citations = 6; WoS Citations = 5; Scopus SNIP (Source Normalised Impact per Paper): 2005 = 0.48; WoS Impact Factor: 2006 = 0.148 (2) - 2007=0.251 (5)
Citation count*12 (2020-01-16)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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