16S rRNA gene‐based metagenomic analysis of the gut microbial community associated with the DUI species Unio crassus (Bivalvia: Unionidae)

Monika Mioduchowska , Katarzyna Zając , Krzysztof Bartoszek , Piotr Madanecki , Jarosław Kur , Tadeusz Zając

Abstract

What factors determine biome richness: genetic or environmental? Sex, phylogeny, and tolerance indicated by other symbionts (e.g., endosymbionts) or simply is it related to local habitat, especially if the gut biome is considered? To answer these questions, we investigated the gut microbial profile of both sexes of three Unio crassus populations, species with unique system of mitochondrial DNA inheritance called doubly uniparental inheritance (DUI), living in different ecological conditions. High‐throughput sequencing of the V3–V4 hypervariable regions in the bacterial 16S rRNA gene fragment was performed, which resulted in a total of 1,051,647 reads, with 58,424 reads/65 OTUs (operational taxonomic units) per sample on average. We identified a core microbiome, with all individual mussels sharing 69 OTUs (representing 23% of the total number of OTUs). Proteobacteria was the dominant phylum in all samples, followed by Firmicutes, Actinobacteria, and Bacteroidetes. There were no significant differences in gut microbiome compositions between the two sexes of this species; however, we observed different phyla in geographically isolated populations. A non‐metric multidimensional scaling plot and dendrogram showed that the bacterial profile complies with the genetic structure of populations. Although we found differences in microbiomes between populations, their genetic structure suggests that the microbiome is weakly related to habitat, and more strongly to phylogeography (on both F and M mitotypes). We found no significant differences in beta diversity between the individuals of the bacterial communities measured using the Bray–Curtis index. Finally, we also examined whether OTUs were represented by symbiotic bacteria that enable cellulose digestion and by endosymbiotic bacteria that play important functions in the biology of their hosts and also affect microevolutionary processes and population phenomena. With regard to the endosymbionts, however, there was no relation to sex of the studied individuals, which suggests that there are no straightforward relations between DUI and microbiome.
Publication typeIn press (online first, early view)
Author Monika Mioduchowska (FB/DGB)
Monika Mioduchowska,,
- Katedra Genetyki i Biosystematyki
, Katarzyna Zając
Katarzyna Zając,,
-
, Krzysztof Bartoszek
Krzysztof Bartoszek,,
-
, Piotr Madanecki
Piotr Madanecki,,
-
, Jarosław Kur
Jarosław Kur,,
-
, Tadeusz Zając
Tadeusz Zając,,
-
Journal seriesJournal of Zoological Systematics and Evolutionary Research, ISSN 0947-5745, e-ISSN 1439-0469, (N/A 70 pkt)
Issue year2020
Noonline first
Pages1-9
Publication size in sheets0.30
Keywords in Englishfreshwater mussels, gut microbiome, next-generation sequencing, operational taxonomic units (OTUs) assignment, Unionidae
ASJC Classification1103 Animal Science and Zoology; 1105 Ecology, Evolution, Behavior and Systematics; 1311 Genetics; 1312 Molecular Biology
DOIDOI:10.1111/jzs.12377
URL https://doi.org/10.1111/jzs.12377
Languageen angielski
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 16-04-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.297; WoS Impact Factor: 2018 = 2.268 (2) - 2018=2.553 (5)
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