Dolichospermum and Aphanizomenon as neurotoxins producers in some Russian freshwaters

Ekaterina Chernova , Sergey Sidelev , Iana Russkikh , Ekaterina Voyakina , Olga Babanazarova , Roman Romanov , Anton Kotovshchikov , Hanna Mazur-Marzec

Abstract

Last decades, cyanobacterial blooms have been commonly reported in Russia. Among the boom-forming species, potential toxin producers have been identified. The aim of this paper was to study the presence of neurotoxic compounds - saxitoxins and anatoxin-a - in water bodies from different regions of Russia. We also made attempts to identify the neurotoxin-producing genera. The good convergence of the results obtained by light microscopy, PCR and LC-MS/MS analyses indicated the presence of active neurotoxin producing species in all investigated water bodies. Saxitoxin was detected in phytoplankton from 4 water bodies in Central European Russia and West Siberia, including lake and reservoirs used as a source for potable water. The water bodies differed with the respect of saxitoxin producers which belonged to Aphanizomenon and/or Dolichospermum genera. For the first time, we obtained quantitative data on the intracellular saxitoxin concentration in Russian freshwaters using LC-MS/MS. Anatoxin-a was detected only in lakes of Northwestern Russia. In the eutrophic shallow Lower Suzdal Lake, Aphanizomenon was the stated anatoxin-a-producing genus. In the large shallow artificial hypertrophic Sestroretskij Razliv Lake, it was very likely that both dominant species - Aphanizomenon flos-aquae and Dolichospermum planctonicum - were anatoxin-a producers.
Author Ekaterina Chernova
Ekaterina Chernova,,
-
, Sergey Sidelev
Sergey Sidelev,,
-
, Iana Russkikh
Iana Russkikh,,
-
, Ekaterina Voyakina
Ekaterina Voyakina,,
-
, Olga Babanazarova
Olga Babanazarova,,
-
, Roman Romanov
Roman Romanov,,
-
, Anton Kotovshchikov
Anton Kotovshchikov,,
-
, Hanna Mazur-Marzec (FOG / IO / DMarB)
Hanna Mazur-Marzec,,
- Division of Marine Biotechnology
Other language title versions
Journal seriesToxicon, ISSN 0041-0101, (A 25 pkt)
Issue year2017
Vol130
Pages47-55
Publication size in sheets0.5
Keywords in Englishcyanotoxins, LC-MS, PCR, neurotoxin producers, Aphanizomenon, Dolichospermum
DOIDOI:10.1016/j.toxicon.2017.02.016
URL http://dx.doi.org/10.1016/j.toxicon.2017.02.016
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 25.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.927 (2) - 2016=2.382 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back