What makes telomeres unique?

Adam Sieradzan , Paweł Krupa , David J. Wales

Abstract

Telomeres are repetitive nucleotide sequences, which are essential for protecting the termini of chromosomes. Thousands of such repetitions are necessary to maintain the stability of the whole chromosome. Several similar repeated telomeric sequences have been found in different species, but why has nature chosen them? What features do telomeres have in common? In this article, we study the physical properties of human-like (TTAGGG), plant (TTTAGG), insect (TTAGG), and Candida guilermondi (GGTGTAC) telomeres in comparison with seven control, nontelomeric sequences. We used steered molecular dynamics with the nucleic acid united residue (NARES) coarse-grained force field, which we compared with the all-atom AMBER14 force field and experimental data. Our results reveal important features in all of the telomeric sequences, including their exceptionally high mechanical resistance and stability to untangling and stretching, compared to those of nontelomeric sequences. We find that the additional stability of the telomeres comes from their ability to form triplex structures and wrap around loose chains of linear DNA by regrabbing the chain. We find that, with slower pulling speed, regrabbing and triplex formation is more frequent. We also found that some of the sequences can form triplexes experimentally, such as TTTTTCCCC, and can mimic telomeric properties.
Author Adam Sieradzan PMM
Adam Sieradzan,,
- Laboratory of Molecular Modeling
, Paweł Krupa PSP
Paweł Krupa,,
- Laboratory of Simulation of Polymers
, David J. Wales
David J. Wales,,
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Other language title versions
Journal seriesJournal of Physical Chemistry B, ISSN 1520-6106
Issue year2017
Vol121
No10
Pages2207-2219
Publication size in sheets0.6
DOIDOI:10.1021/acs.jpcb.6b08780
URL http://pubs.acs.org/doi/pdf/10.1021/acs.jpcb.6b08780
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.177 (2) - 2016=3.177 (5)
Citation count*0
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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