Dense layer of bacteriophages ordered in alternating electric field and immobilized by surface chemical modification as sensing element for bacteria detection
Łukasz Richter , Krzysztof Bielec , Adam Leśniewski , Marcin Łoś , Jan Paczesny , Robert Hołyst
AbstractFaster and more sensitive environmental monitoring should be developed to face the worldwide problem of bacterial infections. To remedy this issue, we demonstrate a bacteria-sensing element that utilizes dense and ordered layers of bacteriophages specific to the given bacteria strain. We combine (1) the chemical modification of a surface to increase the surface coverage of bacteriophages (2) with an alternating electric field to greatly increase the number of properly oriented bacteriophages at the surface. Usually, in sensing elements, a random orientation of bacteriophages results in steric hindrance, which results in no more than a few percent of all receptors being available. An increased number of properly ordered phages results in the optimal performance of phage receptors, manifesting in up to a 64-fold increase in sensitivity and a limit of detection as low as 100 CFU mL−1. Our sensing elements can be applied for selective, sensitive, and fast (15 min) bacterial detection. A well-studied pair T4 bacteriophage-bacteria Escherichia coli, was used as a model; however, the method could be adapted to prepare bacteriophage-based sensors for detection of a variety of bacterial strains.
|Journal series||ACS Applied Materials & Interfaces, ISSN 1944-8244|
|Publication size in sheets||0.5|
|Keywords in English||T4 bacteriophages, orientation, alternating, electric field, surface modification, biosensor, nonlinear electrophoresis|
|Score|| = 40.0, 26-02-2018, ArticleFromJournal|
= 40.0, 26-02-2018, ArticleFromJournal
|Publication indicators||: 2016 = 7.504 (2) - 2016=7.823 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.