HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin

Tomasz Wenta , Dorota Żurawa-Janicka , Michał Rychłowski , Mirosław Jarząb , Przemysław Glaza , Andrea Lipińska , Krystyna Bieńkowska-Szewczyk , Anna Herman-Antosiewicz , Joanna Skórko-Glonek , Barbara Lipińska


The human HtrA3 protease is involved in placentation, mitochondrial homeostasis, stimulation of apoptosis and proposed to be a tumor suppressor. Molecular mechanisms of the HtrA3 functions are poorly understood and knowledge concerning its cellular targets is very limited. There are two HtrA3 isoforms, the long (HtrA3L) and short (HtrA3S). Upon stress, their N-terminal domains are removed, resulting in the more active ΔN-HtrA3. By pull down and mass spectrometry techniques, we identified a panel of putative ΔN-HtrA3L/S substrates. We confirmed that ΔN-HtrA3L/S formed complexes with actin, β-tubulin, vimentin and TCP1α in vitro and in a cell and partially co-localized with the actin and vimentin filaments, microtubules and TCP1α in a cell. In vitro, both isoforms cleaved the cytoskeleton proteins, promoted tubulin polymerization and displayed chaperone-like activity, with ΔN-HtrA3S being more efficient in proteolysis and ΔN-HtrA3L – in polymerization. TCP1α, essential for the actin and tubulin folding, was directly bound by the ΔN-HtrA3L/S but not cleaved. These results indicate that actin, β-tubulin, vimentin, and TCP1α are HtrA3 cellular partners and suggest that HtrA3 may influence cytoskeleton dynamics. They also suggest different roles of the HtrA3 isoforms and a possibility that HtrA3 protease may also function as a co-chaperone. Significance: The HtrA3 protease stimulates apoptosis and is proposed to be a tumor suppressor and a therapeutic target, however little is known about its function at the molecular level and very few HtrA3 physiological substrates have been identified so far. Furthermore, HtrA3 is the only member of the HtrA family of proteins which, apart from the long isoform possessing the PD and PDZ domains (HtrA3L), has a short isoform (HtrA3S) lacking the PDZ domain. In this work we identified a large panel (about 150) of the tentative HtrA3L/S cellular partners which provides a good basis for further research concerning the HtrA3 function. We have shown that the cytoskeleton proteins actin, β-tubulin and vimentin, and the TCP1α chaperonin are cellular partners of both HtrA3 isoforms. Our findings indicate that HtrA3 may promote destabilization of the actin and vimentin cytoskeleton and suggest that it may influence the dynamics of the microtubule network, with the HtrA3S being more efficient in cytoskeleton protein cleavage and HtrA3L – in tubulin polymerization. Also, we have shown for the first time that HtrA3 has a chaperone-like, holdase activity in vitro - activity typical for co-chaperone proteins. The proposed HtrA3 influence on the cytoskeleton dynamics may be one of the ways in which HtrA3 promotes cell death and affects cancerogenesis. We believe that the results of this study provide a new insight into the role of HtrA3 in a cell and further confirm the notion that HtrA3 should be considered as a target of new anti-cancer therapies.
Author Tomasz Wenta (FB / DB)
Tomasz Wenta,,
- Department of Biochemistry
, Dorota Żurawa-Janicka (FB / DB)
Dorota Żurawa-Janicka,,
- Department of Biochemistry
, Michał Rychłowski (IFB / IB / LVMB)
Michał Rychłowski,,
- Laboratory of Virus Molecular Biology
, Mirosław Jarząb (FB / DB)
Mirosław Jarząb,,
- Department of Biochemistry
, Przemysław Glaza (FB / DB)
Przemysław Glaza,,
- Department of Biochemistry
, Andrea Lipińska (IFB / IB / LVMB)
Andrea Lipińska,,
- Laboratory of Virus Molecular Biology
, Krystyna Bieńkowska-Szewczyk (IFB / IB / LVMB)
Krystyna Bieńkowska-Szewczyk,,
- Laboratory of Virus Molecular Biology
, Anna Herman-Antosiewicz (FB / DMBG)
Anna Herman-Antosiewicz,,
- Katedra Biologii i Genetyki Medycznej
, Joanna Skórko-Glonek (FB / DB)
Joanna Skórko-Glonek,,
- Department of Biochemistry
, Barbara Lipińska (FB / DB)
Barbara Lipińska,,
- Department of Biochemistry
Journal seriesJournal of Proteomics, ISSN 1874-3919, (A 35 pkt)
Issue year2018
Publication size in sheets1.15
Keywords in EnglishHtrA proteins, human HtrA3 protease, human HtrA3 chaperone, HtrA3 isoforms, regulation of cytoskeleton dynamics
ASJC Classification1303 Biochemistry; 1304 Biophysics
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, 04-04-2019, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 04-04-2019, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.982; WoS Impact Factor: 2017 = 3.722 (2) - 2017=3.725 (5)
Citation count*
Share Share

Get link to the record

* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.