Combination of field amplified sample injection and hydrophobic interaction electrokinetic chromatography (FASI-HIEKC) as a signal amplification method for the determination of selected macrocyclic antibiotics

Piotr Kowalski , Ilona Olędzka , Alina Plenis , Natalia Miękus , Michał Pieckowski , Tomasz Bączek


In this study, a field amplified sample injection (FASI) and hydrophobic interaction electrokinetic chromatography (HIEKC) method has been developed for the separation of five macrolide antibiotics: spiramycin, ivermectin, tylosin, josamycin, rapamycin, and one ansamycin drug – rifamycin. By the manipulation of both the sample and separation buffer compositions, their pH values and molarity, a systematic approach has been achieved to maximize analyte differential electrophoretic mobility and signal amplification. The impact of the sample solution composition and the injection mode on the signal amplification effect of the six tested antibiotics was also investigated. Moreover, the influence of the injection of the sample and the water plug on the quantity, symmetry and height of the analyte signal was demonstrated. All the analytes were completely resolved in less than 8 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D.) x 50 cm length. The electrophoretic separations were performed in a 60% (v/v) acetonitrile and 20 mM phosphate electrolyte system (pH 7.1) with an applied voltage of 25 kV. The established method was validated and confirmed to be applicable for the determination of the active ingredients in a quality control analysis.
Author Piotr Kowalski
Piotr Kowalski,,
, Ilona Olędzka
Ilona Olędzka,,
, Alina Plenis
Alina Plenis,,
, Natalia Miękus (FB / DAHP)
Natalia Miękus,,
- Department of Animal and Human Physiology
, Michał Pieckowski
Michał Pieckowski,,
, Tomasz Bączek
Tomasz Bączek,,
Journal seriesAnalytica Chimica Acta, ISSN 0003-2670, (N/A 100 pkt)
Issue year2019
Publication size in sheets0.5
Keywords in Englishsignal enhancement technique, hydrophobic interaction electrokinetic, chromatography, field amplified sample injection, macrolide and ansamycin antibiotics
ASJC Classification1607 Spectroscopy; 2304 Environmental Chemistry; 1303 Biochemistry; 1602 Analytical Chemistry
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 28-01-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.386; WoS Impact Factor: 2018 = 5.256 (2) - 2018=5.174 (5)
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