Transcriptome analysis of rainbow trout (Oncorhynchus mykiss) eggs subjected to the high hydrostatic pressure treatment

Artur Gurgul , Klaudia Pawlina-Tyszko , Monika Bugno-Poniewierska , Tomasz Szmatoła , Igor Jasielczuk , Stefan Dobosz , Konrad Ocalewicz

Abstract

High hydrostatic pressure (HHP) causes depolymerization of the spindle microtubules. HHP applied to fish eggs prevents extrusion of the second polar body and inhibits the first cell cleavage, and it is used to produce triploids and diploid gynogenetic and androgenetic individuals. HHP has been also found to affect biomolecules including nucleic acids, and it may be presumed that HHP administered to the rainbow trout (Oncorhynchus mykiss) eggs disturbs cytoplasmic maternal RNA indispensable for the early embryogenesis. To verify this assumption, quality and quantity of RNA extracted from the rainbow trout eggs subjected to the high hydrostatic pressure shock were analyzed. Provided results exhibited that maternal transcriptome was resistant to a three-minute exposure to 65.5 MPa of HHP treatment. Some trend showing increase of the RNA integrity was observed in the HHP-treated eggs; however, the difference was not statistically significant. Alterations in the expression profiles in the rainbow trout eggs subjected to HHP were also negligible. Greater differences in the maternal gene expression were observed between eggs from different clutches than between HHP-treated and untreated eggs from the same clutch. It may be assumed that exposure to HHP shock was too short to modify significantly maternal transcripts in the rainbow trout eggs.
Author Artur Gurgul
Artur Gurgul,,
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, Klaudia Pawlina-Tyszko
Klaudia Pawlina-Tyszko,,
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, Monika Bugno-Poniewierska
Monika Bugno-Poniewierska,,
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, Tomasz Szmatoła
Tomasz Szmatoła,,
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, Igor Jasielczuk
Igor Jasielczuk,,
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, Stefan Dobosz
Stefan Dobosz,,
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, Konrad Ocalewicz (FOG / IO / DMBE)
Konrad Ocalewicz,,
- Department of Marine Biology and Ecology
Journal seriesInternational Journal of Genomics, ISSN 2314-436X, (A 20 pkt)
Issue year2018
Vol2018
Pages1-7
Publication size in sheets0.5
DOIDOI:10.1155/2018/5197126
URL http://downloads.hindawi.com/journals/ijg/2018/5197126.pdf
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)20
ScoreMinisterial score = 20.0, 04-09-2018, ArticleFromJournal
Ministerial score (2013-2016) = 20.0, 04-09-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.402 (2) - 2016=2.067 (5)
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