Induced pluripotent stem cells derived from rabbits exhibit some characteristics of naïve pluripotency

Pierre Osteil , Yann Tapponnier , Suzy Markossian , Murielle Godet , Barbara Schmaltz-Panneau , Luc Jouneau , Cédric Cabau , Thierry Joly , Thierry Blachère , Elen Gócza , Agnieszka Bernat , Martine Yerle , Hervé Acloque , Sullivan Hidot , Zsuzsanna Bosze , Véronique Duranthon , Pierre Savatier , Marielle Afanassieff


Not much is known about the molecular and functional features of pluripotent stem cells (PSCs) in rabbits. To address this, we derived and characterized 2 types of rabbit PSCs from the same breed of New Zealand White rabbits: 4 lines of embryonic stem cells (rbESCs), and 3 lines of induced PSCs (rbiPSCs) that were obtained by reprogramming adult skin fibroblasts. All cell lines required fibroblast growth factor 2 for their growth and proliferation. All rbESC lines showed molecular and functional properties typically associated with primed pluripotency. The cell cycle of rbESCs had a prolonged G1 phase and a DNA damage checkpoint before entry into the S phase, which are the 2 features typically associated with the somatic cell cycle. In contrast, the rbiPSC lines exhibited some characteristics of naı¨ve pluripotency, including resistance to single-cell dissociation by trypsin, robust activity of the distal enhancer of the mouse Oct4 gene, and expression of naïve pluripotency-specific genes, as defined in rodents. According to gene expression profiles, rbiPSCs were closer to the rabbit inner cell mass (ICM) than rbESCs. Furthermore, rbiPSCs were capable of colonizing the ICM after aggregation with morulas. Therefore, we propose that rbiPSCs self-renew in an intermediate state between naı¨ve and primed pluripotency, which represents a key step toward the generation of bona fide naı¨ve PSC lines in rabbits.
Author Pierre Osteil
Pierre Osteil,,
, Yann Tapponnier
Yann Tapponnier,,
, Suzy Markossian
Suzy Markossian,,
, Murielle Godet
Murielle Godet,,
, Barbara Schmaltz-Panneau
Barbara Schmaltz-Panneau,,
, Luc Jouneau
Luc Jouneau,,
, Cédric Cabau
Cédric Cabau,,
, Thierry Joly
Thierry Joly,,
, Thierry Blachère
Thierry Blachère,,
, Elen Gócza
Elen Gócza,,
et al.`
Journal seriesBiology Open, ISSN 2046-6390, (A 35 pkt)
Issue year2013
Publication size in sheets0.75
Keywords in Englishrabbit model, embryonic stem cell, induced pluripotent stem cell, pluripotency, cell cycle
ASJC Classification1100 General Agricultural and Biological Sciences; 1300 General Biochemistry, Genetics and Molecular Biology
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa - Użycie Niekomercyjne - Na tych samych warunkach (CC-BY-NC-SA); with publication
Not used for evaluationyes
Score (nominal)0
Publication indicators WoS Citations = 19; Scopus SNIP (Source Normalised Impact per Paper): 2014 = 0.084; WoS Impact Factor: 2014 = 2.416 (2) - 2014=2.432 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.