A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test

Lucyna Holec-Gąsior , Bartłomiej Ferra , Justyna Czechowska , Illia Serdiuk , Karol Krzymiński

Abstract

Toxoplasma gondii infection is one of the most common human zoonosis. Laboratory diagnosis of this disease is mainly based on the results of serological methods detecting specific antibodies in the patient’s sera. In this study we aimed to evaluate the performance of a chemiluminescence immunoassay (CLIA) based on the use of a novel immunochemical reagent in the form of the conjugate of original acridinium label (AL) attached to secondary antibody (IgG-AL) and SAG2-GRA1-ROP1L chimeric antigen for T. gondii specific antibodies detection. The CLIA test was compared with conventional ELISA, which was based on the same recombinant antigen and differed only in terms of the detectionmethodology of immune complexes. The newCLIA assay proved tobemore sensitiveandbetter differentiated sera of patientswith T. gondii infection fromsera of healthy individuals, being a promising alternative to more labor, cost-demanding and less versatile ELISA as screening test in toxoplasmosis diagnostics.
Author Lucyna Holec-Gąsior
Lucyna Holec-Gąsior,,
-
, Bartłomiej Ferra
Bartłomiej Ferra,,
-
, Justyna Czechowska (FCh / DPCh / LLR)
Justyna Czechowska,,
- Laboratory of Luminescence Research
, Illia Serdiuk (FMPI / IEP)
Illia Serdiuk,,
- Institute of Experimental Physics
, Karol Krzymiński (FCh / DPCh / LLR)
Karol Krzymiński,,
- Laboratory of Luminescence Research
Journal seriesDiagnostic Microbiology and Infectious Disease, ISSN 0732-8893, (A 25 pkt)
Issue year2018
Vol91
No1
Pages13-19
Publication size in sheets1.15
Keywords in EnglishCLIA, ELISA, serological detection, chimeric antigen, chemiluminescence, acridinium esters
DOIDOI:10.1016/j.diagmicrobio.2017.12.022
URL https://doi.org/10.1016/j.diagmicrobio.2017.12.022
Languageen angielski
Score (nominal)25
ScoreMinisterial score = 25.0, 09-02-2018, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 09-02-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.401 (2) - 2016=2.488 (5)
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