Pro-apoptotic action of protein-carbohydrate fraction isolated from coelomic fluid of the earthworm Dendrobaena veneta against human colon adenocarcinoma cells

Arkadiusz Czerwonka , Marta J. Fiołka , Katarzyna Jędrzejewska , Elżbieta Jankowska , Adrian Zając , Wojciech Rzeski

Abstract

Earthworm coelomic fluid (CF) is known as a rich source of various bioactive compounds with promising anticancer features. However, it has been demonstrated that CF affects functionality of both, cancer and normal cells. This non-selective activity causes a major problem for medical application of CF. In this study, we present the anticancer activity of the active protein-carbohydrate fraction (AF) isolated from thermally treated CF of earthworm Dendrobaena veneta. The in vitro effect of the AF was examined in human colon model including normal human colon epithelium (CCD 841 CoTr) and human colon adenocarcinoma (HT-29 and LS180) cell lines. We investigated the impact of AF on cell viability neutral red and lactate dehydrogenase assays, morphology May-Grünwald-Giemsa staining assay proliferation MTT tetrazolium salt and BrdU incorporation assays as well as cell cycle progression propidium iodide/RNase staining and the activity of human 20S proteasome the hydrolysis of AMC from a Suc-LLVY-AMC peptide substrate. Additionally, the influence of AF on apoptosis was examined in HT-29 cells by Annexin V/PI, Hoechst 33342 staining and active caspase-3 assays. Our investigation demonstrated that AF at the tested concentration range does not affect the viability and morphology of CCD 841 CoTr cells. Simultaneously, AF inhibits human 20S proteasome activity as well as significantly decreases mitochondrial metabolism, disturbs cell cycle and induces apoptosis via activation of procaspase-3 in HT-29 cancer cells. Obtained results demonstrate the antiproliferative and proapoptotic activity of AF that can be useful in developing therapeutic strategies to treat human colon cancer.
Author Arkadiusz Czerwonka
Arkadiusz Czerwonka,,
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, Marta J. Fiołka
Marta J. Fiołka,,
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, Katarzyna Jędrzejewska (FCh / DBCh / LMCh)
Katarzyna Jędrzejewska,,
- Laboratory of Medical Chemistry
, Elżbieta Jankowska (FCh / DBCh / LMCh)
Elżbieta Jankowska,,
- Laboratory of Medical Chemistry
, Adrian Zając
Adrian Zając,,
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, Wojciech Rzeski
Wojciech Rzeski,,
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Journal seriesBiomedicine & Pharmacotherapy, [Biomedicine and Pharmacotherapy], ISSN 0753-3322, e-ISSN 1950-6007, (N/A 100 pkt)
Issue year2020
Vol126
Pages1-8
Publication size in sheets0.5
Article number110035
Keywords in EnglishEarthworms, coelomic fluid, Dendrobaena veneta, coon cancer, apoptosis
ASJC Classification2700 General Medicine; 3004 Pharmacology
DOIDOI:10.1016/j.biopha.2020.110035
Languageen angielski
LicenseRepository; published final; Uznanie Autorstwa (CC-BY); with publication
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Czerwonka_Arkadiusz_Pro_apoptopic_action_2020.pdf 2.74 MB
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 02-04-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.951; WoS Impact Factor: 2018 = 3.743 (2) - 2018=3.615 (5)
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LicencjaUtwór jest udostępniany na licencji Creative Commons Uznanie autorstwa 4.0 Międzynarodowe (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/d
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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