Cytogenetic identification of genomic elements of brook trout (Salvelinus fontinalis) in the karyotype of Arctic char (Salvelinus alpinus) from the aquaculture broodstock

Konrad Pomianowski , Małgorzata Jankun , Konrad Ocalewicz

Abstract

Growing interest of Arctic char (Salvelinus alpinus) aquaculture in Europe, and the fact that it can easily hybridize with brook trout (Salvelinus fontinalis) resulting in fertile progeny, led us to investigate fish from the farmed stocks. Chromosomes of sampled Arctic char were examined using conventional and molecular cytogenetic (FISH) techniques in order to determine possible contamination of genomic elements of brook trout. Investigated fish possessed karyotypes composed of 80–82 chromosomes and up to three chromosome fragments. Using staining methods and FISH approach enabled identification of the brook trout chromosomes in the eight out of twenty‐two examined Arctic char. Specific location of AT‐, GC‐ positive and NOR sites observed on chromosomes as well as chromosome fragments in the karyotypes of several individuals points on past chromosomal rearrangements in fish from examined broodstock. Based on our results, it may be assumed that individuals with the brook trout genomic elements, although phenotypically identified as Arctic chars, were hybrids. Our results highlights that special care should be taken to protect gene pools of brook trout and Arctic char in farms where both species are cultured.
Publication typeIn press (online first, early view)
Author Konrad Pomianowski
Konrad Pomianowski,,
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, Małgorzata Jankun
Małgorzata Jankun,,
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, Konrad Ocalewicz (FOG / IO / DMBE)
Konrad Ocalewicz,,
- Department of Marine Biology and Ecology
Journal seriesJournal of Applied Ichthyology, ISSN 0175-8659, (N/A 40 pkt)
Issue year2019
Noonline first
Pages1-7
Publication size in sheets0.5
Keywords in Englishaquaculture of salmonids, Arctic char, chromosome rearrangements, hybridization, interstitial telomeric sequences, karyotype variation
ASJC Classification1104 Aquatic Science
DOIDOI:10.1111/jai.13913
URL https://onlinelibrary.wiley.com/doi/epdf/10.1111/jai.13913
Languageen angielski
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 17-11-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.915; WoS Impact Factor: 2018 = 0.877 (2) - 2018=1.034 (5)
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