Demonstration of FRET in solutions

Sunil Shah , Zygmunt Gryczynski , Rahul Chib , Rafał Fudala , Aatmun Baxi , Julian Borejdo , Anna Synak , Ignacy Gryczyński

Abstract

We measured the Förster resonance energy transfer (FRET) from Uranin (U) donor to Rhodamine 101(R101) acceptor in propylene glycol. Steady-state fluorescence measurements show a significant difference between mixed and unmixed fluorophore solutions. In the solution with mixed fluorophores, fluorescence intensity of the U donor decreases and intensity of R101 fluorescence increases. This is visualized as a color change from green to orange. Fluorescence anisotropy of the mixture solution increases in the donor emission wavelength region and decreases in the acceptor emission wavelengths; which is consistent with FRET occurrence. Time-resolved (lifetime) measurements show a decrease of the U lifetime in the presence of R101 acceptor. In the intensity decay of R101 acceptor appears a negative component indicating excited state process. All these measurements prove the presence of FRET in U/R101 mixture fluorescence.
Author Sunil Shah
Sunil Shah,,
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, Zygmunt Gryczynski
Zygmunt Gryczynski,,
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, Rahul Chib
Rahul Chib,,
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, Rafał Fudala
Rafał Fudala,,
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, Aatmun Baxi
Aatmun Baxi,,
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, Julian Borejdo
Julian Borejdo,,
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, Anna Synak IFD
Anna Synak,,
- Institute of Experimental Physics
, Ignacy Gryczyński
Ignacy Gryczyński,,
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Journal seriesMethods and Applications in Fluorescence, ISSN 2050-6120
Issue year2016
Vol4
No1
Pages1-9
Publication size in sheets0.5
Keywords in Englishfluorescence, 3D FRET, FRET in solution, Forster distance
DOIDOI:10.1088/2050-6120/4/1/015001
URL http://iopscience.iop.org/article/10.1088/2050-6120/4/1/015001/pdf
Languageen angielski
Score (nominal)25
ScoreMinisterial score = 25.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.656 (2) - 2016=2.765 (5)
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