Demonstration of FRET in solutions
Sunil Shah , Zygmunt Gryczynski , Rahul Chib , Rafał Fudala , Aatmun Baxi , Julian Borejdo , Anna Synak , Ignacy Gryczyński
AbstractWe measured the Förster resonance energy transfer (FRET) from Uranin (U) donor to Rhodamine 101(R101) acceptor in propylene glycol. Steady-state fluorescence measurements show a significant difference between mixed and unmixed fluorophore solutions. In the solution with mixed fluorophores, fluorescence intensity of the U donor decreases and intensity of R101 fluorescence increases. This is visualized as a color change from green to orange. Fluorescence anisotropy of the mixture solution increases in the donor emission wavelength region and decreases in the acceptor emission wavelengths; which is consistent with FRET occurrence. Time-resolved (lifetime) measurements show a decrease of the U lifetime in the presence of R101 acceptor. In the intensity decay of R101 acceptor appears a negative component indicating excited state process. All these measurements prove the presence of FRET in U/R101 mixture fluorescence.
|Journal series||Methods and Applications in Fluorescence, ISSN 2050-6120|
|Publication size in sheets||0.5|
|Keywords in English||fluorescence, 3D FRET, FRET in solution, Forster distance|
|Score|| = 25.0, 20-12-2017, ArticleFromJournal|
= 25.0, 20-12-2017, ArticleFromJournal
|Publication indicators||: 2016 = 2.656 (2) - 2016=2.765 (5)|
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