Induced androgenetic development in rainbow trout and transcriptome analysis of irradiated eggs

Konrad Ocalewicz , Artur Gurgul , Klaudia Pawlina-Tyszko , Tomasz Szmatoła , Igor Jasielczuk , Monika Bugno-Poniewierska , Stefan Dobosz

Abstract

Ionizing radiation is administered to damage nuclear genome in fish eggs during induced androgenesis. In this study, we examined whether 350 Gy of X-ray applied to damage chromosomes in the rainbow trout eggs affects maternal RNA. Shortly after irradiation, we did not find any symptoms of RNA degradation in the treated eggs. Significant (p < 0.01) differences between non-irradiated and irradiated eggs concerned only a few transcripts including increased expression of immediate early response 2 (IER2) and early growth response 1 (EGR1) genes observed in the irradiated eggs. Both genes belong to the group of "immediate early genes" that respond quickly to the diverse extracellular stimuli. Elevated expression of these genes was accompanied by decreased level of ssa-miR-10b-5p and ssa-miR-21b-5p (p < 0.05), for which IER2 and EGR1 are target genes. The level of RNA in the fertilized irradiated eggs was highly significantly lower than in the non-irradiated eggs (p < 0.001) and in the unfertilized irradiated eggs (p < 0.0001). However, transcriptome profiles of fertilized non-irradiated eggs and fertilized irradiated eggs did not differ significantly. Thus, we assume that reduced abundance of mRNA in the fertilized irradiated eggs was associated with post-translational degradation and clearance of the maternal transcripts rather than from the irradiation of eggs.
Author Konrad Ocalewicz (FOG / IO / DMBE)
Konrad Ocalewicz,,
- Department of Marine Biology and Ecology
, Artur Gurgul
Artur Gurgul,,
-
, Klaudia Pawlina-Tyszko
Klaudia Pawlina-Tyszko,,
-
, Tomasz Szmatoła
Tomasz Szmatoła,,
-
, Igor Jasielczuk
Igor Jasielczuk,,
-
, Monika Bugno-Poniewierska
Monika Bugno-Poniewierska,,
-
, Stefan Dobosz
Stefan Dobosz,,
-
Journal seriesScientific Reports, ISSN 2045-2322, (A 40 pkt)
Issue year2019
Vol9
Pages1-12
Publication size in sheets0.55
Article number 8084
ASJC Classification1000 Multidisciplinary
DOIDOI:10.1038/s41598-019-44568-7
URL https://www.nature.com/articles/s41598-019-44568-7.pdf
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 40.0, 24-07-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.401; WoS Impact Factor: 2017 = 4.122 (2) - 2017=4.609 (5)
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