Mitochondrial DNA levels in Huntington disease leukocytes and dermal fibroblasts
Paulina Jędrak , Magdalena Krygier , Katarzyna Tońska , Małgorzata Drozd , Magdalena Kaliszewska , Ewa Bartnik , Witold Sołtan , Emilia J. Sitek , Anna Stanisławska-Sachadyn , Janusz Limon , Jarosław Sławek , Grzegorz Węgrzyn , Sylwia Barańska
AbstractHuntington disease (HD) is an inherited neurodegenerative disorder caused by mutations in the huntingtin gene. Involvement of mitochondrial dysfunctions in, and especially influence of the level of mitochondrial DNA (mtDNA) on, development of this disease is unclear. Here, samples of blood from 84 HD patients and 79 controls, and dermal fibroblasts from 10 HD patients and 9 controls were analysed for mtDNA levels. Although the type of mitochondrial haplogroup had no influence on the mtDNA level, and there was no correlation between mtDNA level in leukocytes in HD patients and various parameters of HD severity, some considerable differences between HD patients and controls were identified. The average mtDNA/nDNA relative copy number was significantly higher in leukocytes, but lower in fibroblasts, of symptomatic HD patients relative to the control group. Moreover, HD women displayed higher mtDNA levels in leukocytes than HD men. Because this is the largest population analysed to date, these results might contribute to explanation of discrepancies between previously published studies concerning levels of mtDNA in cells of HD patients. We suggest that the size of the investigated population and type of cells from which DNA is isolated could significantly affect results of mtDNA copy number estimation in HD. Hence, these parameters should be taken into consideration in studies on mtDNA in HD, and perhaps also in other diseases where mitochondrial dysfunction occurs.
|Journal series||Metabolic Brain Disease, ISSN 0885-7490, (A 20 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||huntington disease, mitochondrial DNA, leukocytes, dermal fibroblasts, haplogroup|
|ASJC Classification||; ;|
|License||Other; published final; ; with publication|
|Score||= 20.0, 24-07-2019, ArticleFromJournal|
|Publication indicators||= 6; : 2016 = 0.798; : 2017 = 2.441 (2) - 2017=2.481 (5)|
|Citation count*||11 (2019-08-16)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.