Antibacterial activity of fructose-stabilized silver nanoparticles produced by direct current atmospheric pressure glow discharge towards quarantine pests

Anna Dzimitrowicz , Agata Motyka-Pomagruk , Piotr Cyganowski , Weronika Babińska , Dominik Terefinko , Piotr Jamroz , Ewa Łojkowska , Paweł Pohl , Wojciech Śledź

Abstract

Development of efficient plant protection methods against bacterial phytopathogens subjected to compulsory control procedures under international legislation is of the highest concern having in mind expensiveness of enforced quarantine measures and threat of the infection spread in disease-free regions. In this study, fructose-stabilized silver nanoparticles (FRU-AgNPs) were produced using direct current atmospheric pressure glow discharge (dc-APGD) generated between the surface of a flowing liquid anode (FLA) solution and a pin-type tungsten cathode in a continuous flow reaction-discharge system. Resultant spherical and stable in time FRU-AgNPs exhibited average sizes of 14.9 ± 7.9 nm and 15.7 ± 2.0 nm, as assessed by transmission electron microscopy (TEM) and dynamic light scattering (DLS), respectively. Energy dispersive X-ray spectroscopy (EDX) analysis revealed that the obtained nanomaterial was composed of Ag while selected area electron diffraction (SAED) indicated that FRU-AgNPs had the face-centered cubic crystalline structure. The fabricated FRU-AgNPs show antibacterial properties against Erwinia amylovora, Clavibacter michiganensis, Ralstonia solanacearum, Xanthomonas campestris pv. campestris and Dickeya solani strains with minimal inhibitory concentrations (MICs) of 1.64 to 13.1 mg L−1 and minimal bactericidal concentrations (MBCs) from 3.29 to 26.3 mg L−1. Application of FRU-AgNPs might increase the repertoire of available control procedures against most devastating phytopathogens and as a result successfully limit their agricultural impact.
Author Anna Dzimitrowicz
Anna Dzimitrowicz,,
-
, Agata Motyka-Pomagruk (IFB)
Agata Motyka-Pomagruk,,
- Intercollegiate Faculty of Biotechnology UG
, Piotr Cyganowski
Piotr Cyganowski,,
-
, Weronika Babińska (IFB)
Weronika Babińska,,
- Intercollegiate Faculty of Biotechnology UG
, Dominik Terefinko
Dominik Terefinko,,
-
, Piotr Jamroz
Piotr Jamroz,,
-
, Ewa Łojkowska (IFB / IB / DBiotech)
Ewa Łojkowska,,
- Department of Biotechnology
, Paweł Pohl
Paweł Pohl,,
-
, Wojciech Śledź (IFB / IB / DBiotech)
Wojciech Śledź,,
- Department of Biotechnology
Journal seriesNanomaterials, ISSN 2079-4991, (A 35 pkt)
Issue year2018
Vol8
No10
Pages1-16
Keywords in Englishatmospheric pressure plasma, nanostructures, phytopathogens, plant protection, quarantine, Erwinia amylovora, Clavibacter michiganensis, Ralstonia solanacearum, Xanthomonas campestris pv. campestris, Dickeya solani
DOIDOI:10.3390/nano8100751
URL https://doi.org/10.3390/nano8100751
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)35
ScoreMinisterial score = 35.0, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, ArticleFromJournal
Publication indicators WoS Impact Factor: 2017 = 3.504 (2) - 2017=3.811 (5)
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