Antibacterial activity of fructose-stabilized silver nanoparticles produced by direct current atmospheric pressure glow discharge towards quarantine pests

Anna Dzimitrowicz , Agata Motyka-Pomagruk , Piotr Cyganowski , Weronika Babińska , Dominik Terefinko , Piotr Jamroz , Ewa Łojkowska , Paweł Pohl , Wojciech Śledź

Abstract

Development of efficient plant protection methods against bacterial phytopathogens subjected to compulsory control procedures under international legislation is of the highest concern having in mind expensiveness of enforced quarantine measures and threat of the infection spread in disease-free regions. In this study, fructose-stabilized silver nanoparticles (FRU-AgNPs) were produced using direct current atmospheric pressure glow discharge (dc-APGD) generated between the surface of a flowing liquid anode (FLA) solution and a pin-type tungsten cathode in a continuous flow reaction-discharge system. Resultant spherical and stable in time FRU-AgNPs exhibited average sizes of 14.9 ± 7.9 nm and 15.7 ± 2.0 nm, as assessed by transmission electron microscopy (TEM) and dynamic light scattering (DLS), respectively. Energy dispersive X-ray spectroscopy (EDX) analysis revealed that the obtained nanomaterial was composed of Ag while selected area electron diffraction (SAED) indicated that FRU-AgNPs had the face-centered cubic crystalline structure. The fabricated FRU-AgNPs show antibacterial properties against Erwinia amylovora, Clavibacter michiganensis, Ralstonia solanacearum, Xanthomonas campestris pv. campestris and Dickeya solani strains with minimal inhibitory concentrations (MICs) of 1.64 to 13.1 mg L−1 and minimal bactericidal concentrations (MBCs) from 3.29 to 26.3 mg L−1. Application of FRU-AgNPs might increase the repertoire of available control procedures against most devastating phytopathogens and as a result successfully limit their agricultural impact.
Author Anna Dzimitrowicz - [Wrocław University of Science and Technology]
Anna Dzimitrowicz,,
-
- Wrocław University of Science and Technology
, Agata Motyka-Pomagruk (IFB)
Agata Motyka-Pomagruk,,
- Intercollegiate Faculty of Biotechnology UG
, Piotr Cyganowski - [Wrocław University of Science and Technology]
Piotr Cyganowski,,
-
- Wrocław University of Science and Technology
, Weronika Babińska (IFB)
Weronika Babińska,,
- Intercollegiate Faculty of Biotechnology UG
, Dominik Terefinko - [Wrocław University of Science and Technology]
Dominik Terefinko,,
-
- Wrocław University of Science and Technology
, Piotr Jamroz - [Wrocław University of Science and Technology]
Piotr Jamroz,,
-
- Wrocław University of Science and Technology
, Ewa Łojkowska (IFB/IB/DBiotech)
Ewa Łojkowska,,
- Department of Biotechnology
, Paweł Pohl - [Wrocław University of Science and Technology]
Paweł Pohl,,
-
- Wrocław University of Science and Technology
, Wojciech Śledź (IFB/IB/DBiotech)
Wojciech Śledź,,
- Department of Biotechnology
Journal seriesNanomaterials, ISSN 2079-4991, (A 35 pkt)
Issue year2018
Vol8
No10
Pages1-16
Publication size in sheets0.75
Article number751
Keywords in Englishatmospheric pressure plasma, nanostructures, phytopathogens, plant protection, quarantine, Erwinia amylovora, Clavibacter michiganensis, Ralstonia solanacearum, Xanthomonas campestris pv. campestris, Dickeya solani
DOIDOI:10.3390/nano8100751
URL https://doi.org/10.3390/nano8100751
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)35
Score sourcejournalList
ScoreMinisterial score = 35.0, 24-07-2020, ArticleFromJournal
Publication indicators WoS Citations = 4.000; Scopus Citations = 7.000; WoS Impact Factor: 2018 = 4.034 (2) - 2018=4.358 (5)
Citation count*8 (2020-08-01)
Cite
busy
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?