High-titre neutralizing antibodies to H1N1 influenza virus after mouse immunization with yeast expressed H1 antigen: a promising influenza vaccine candidate

Edyta Kopera , Konrad Zdanowski , Karolina Uranowska , Piotr Kosson , Violetta Sączyńska , Katarzyna Florys , Bogusław Szewczyk

Abstract

H1N1 influenza virus is still regarded as a serious pandemic threat. The most effective method of protection against influenza virus and the way to reduce the risk of epidemic or pandemic spread is vaccination. Influenza vaccine manufactured in a traditional way, though well developed, has some drawbacks and limitations which have stimulated interest in developing alternative approaches. In this study, we demonstrate that the recombinant H1 vaccine based on the hydrophilic haemagglutinin (HA) domain and produced in the yeast system elicited high titres of serum haemagglutination-inhibiting antibodies in mice. Transmission electron microscopy showed that H1 antigen oligomerizes into functional higher molecular forms similar to rosette-like structures. Analysis of the N-linked glycans using mass spectrometry revealed that the H1 protein is glycosylated at the same sites as the native HA. The recombinant antigen was secreted into a culture medium reaching approximately 10 mg/l. These results suggest that H1 produced inPichia pastoriscan be considered as the vaccine candidate against H1N1 virus.
Author Edyta Kopera
Edyta Kopera,,
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, Konrad Zdanowski
Konrad Zdanowski,,
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, Karolina Uranowska (IFB)
Karolina Uranowska,,
- Intercollegiate Faculty of Biotechnology UG
, Piotr Kosson
Piotr Kosson,,
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, Violetta Sączyńska
Violetta Sączyńska,,
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, Katarzyna Florys
Katarzyna Florys,,
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, Bogusław Szewczyk (IFB / IB / LRV)
Bogusław Szewczyk,,
- Laboratory of Recombinant Vaccines
Journal seriesJournal of Immunology Research, ISSN 2314-8861, (N/A 100 pkt)
Issue year2019
Vol2019
Pages1-9
Publication size in sheets0.5
Article number2463731
ASJC Classification2403 Immunology; 2700 General Medicine; 2723 Immunology and Allergy
DOIDOI:10.1155/2019/2463731
URL https://doi.org/10.1155/2019/2463731
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 17-11-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.066; WoS Impact Factor: 2018 = 3.404 (2) - 2018=3.738 (5)
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