4-(Methylthio)butyl isothiocyanate inhibits the proliferation of breast cancer cells with different receptor status

Monika Prełowska , Angelika Kaczyńska , Anna Herman-Antosiewicz

Abstract

BackgroundEpidemiological studies indicate that the consumption of Brassicaceae plants, a rich source of biologically active isothiocyanates (ITCs), may effectively reduce cancer risk. In the current study, we evaluated the anticancer potential of 4-(methylthio)butyl ITC (erucin, ERN) against three phenotypically different breast cancer cell lines: MDA-MB-231, SKBR-3 and T47D.MethodsThe effect of ERN on the viability of breast cancer cells was evaluated using sulforhodamine B and clonogenic assays, and acridine orange/ethidium bromide staining. Cell cycle was investigated using flow cytometry. The status of signaling molecules was examined by western blot analysis.ResultsERN decreased the viability of all tested cancer cell lines in a concentration-dependent manner; this effect was much weaker in normal breast cells (MCF-10A). ERN induced cell cycle arrest in the G2/M phase, down-regulated the phosphorylation of S6 ribosomal protein in all tested breast cancer cell lines, and reduced HER2 receptor levels in SKBR-3 cells. A 24-h treatment with lower concentrations of ERN (5–20 μM) induced apoptosis; higher ERN concentrations (40 μM) induced necrosis. The latter also irreversibly inhibited the proliferative potential of cancer cells.ConclusionERN effectively inhibits proliferation of breast cancer cells irrespectively of their receptor status.
Author Monika Prełowska
Monika Prełowska,,
-
, Angelika Kaczyńska (FB / DMBG)
Angelika Kaczyńska,,
- Katedra Biologii i Genetyki Medycznej
, Anna Herman-Antosiewicz (FB / DMBG)
Anna Herman-Antosiewicz,,
- Katedra Biologii i Genetyki Medycznej
Journal seriesPharmacological Reports, ISSN 1734-1140, (A 25 pkt)
Issue year2017
Vol69
No5
Pages1059-1066
Publication size in sheets0.5
Keywords in Englisherucin, isothiocyanates, breast cancer, apoptosis, necrosis
DOIDOI:10.1016/j.pharep.2017.04.014
URL https://doi.org/10.1016/j.pharep.2017.04.014
Languageen angielski
Score (nominal)25
ScoreMinisterial score = 25.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.587 (2) - 2016=2.536 (5)
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