4-(Methylthio)butyl isothiocyanate inhibits the proliferation of breast cancer cells with different receptor status
Monika Prełowska , Angelika Kaczyńska , Anna Herman-Antosiewicz
AbstractBackgroundEpidemiological studies indicate that the consumption of Brassicaceae plants, a rich source of biologically active isothiocyanates (ITCs), may effectively reduce cancer risk. In the current study, we evaluated the anticancer potential of 4-(methylthio)butyl ITC (erucin, ERN) against three phenotypically different breast cancer cell lines: MDA-MB-231, SKBR-3 and T47D.MethodsThe effect of ERN on the viability of breast cancer cells was evaluated using sulforhodamine B and clonogenic assays, and acridine orange/ethidium bromide staining. Cell cycle was investigated using flow cytometry. The status of signaling molecules was examined by western blot analysis.ResultsERN decreased the viability of all tested cancer cell lines in a concentration-dependent manner; this effect was much weaker in normal breast cells (MCF-10A). ERN induced cell cycle arrest in the G2/M phase, down-regulated the phosphorylation of S6 ribosomal protein in all tested breast cancer cell lines, and reduced HER2 receptor levels in SKBR-3 cells. A 24-h treatment with lower concentrations of ERN (5–20 μM) induced apoptosis; higher ERN concentrations (40 μM) induced necrosis. The latter also irreversibly inhibited the proliferative potential of cancer cells.ConclusionERN effectively inhibits proliferation of breast cancer cells irrespectively of their receptor status.
|Journal series||Pharmacological Reports, ISSN 1734-1140, (A 25 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||erucin, isothiocyanates, breast cancer, apoptosis, necrosis|
|Score||= 25.0, 24-07-2019, ArticleFromJournal|
|Publication indicators||: 2016 = 0.983; : 2017 = 2.787 (2) - 2017=2.702 (5)|
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