Acyl-CoA:lysophosphatidylcholine acyltransferases (LPCATs) of Camelina sativa seeds: biochemical properties and function

Sylwia Klińska , Katarzyna Jasieniecka-Gazarkiewicz , Antoni Banaś

Abstract

The transfer of polyunsaturated fatty acids from the location of their synthesis (phosphatidylcholine) to other lipids, e.g., triacylglycerol, remains insufficiently understood. Several enzymes could be involved in this process. One of these enzymes is acyl-CoA:lysophosphatidylcholine acyltransferases (LPCATs). In Camelina sativa seeds, LPCATs could be one of the most important players in this process. Our data clearly indicate that the CsLPCATs present in developing seeds have the potential to transfer almost all polyunsaturated fatty acids synthesised on phosphatidylcholine to the acyl-CoA pool. CsLPCAT activity is the highest at 30 °C, and the enzymes operate well at a pH of 7.0–11.0, with the best activity at a pH of 9.0. The activity of CsLPCATs was inhibited by calcium and magnesium ions at a concentration of 0.05–2 mM. In the forward reaction, CsLPCATs preferentially utilise 18:2-CoA; however, other C18 unsaturated fatty acids are also well accepted. In the backward reactions, there is no clear discrimination between the C18 unsaturated fatty acids utilised by the enzymes for phosphatidylcholine remodelling. The activity of CsLPCATs does not differ much between the stages of seed development.
Author Sylwia Klińska (IFB)
Sylwia Klińska,,
- Intercollegiate Faculty of Biotechnology UG
, Katarzyna Jasieniecka-Gazarkiewicz (IFB / IB / DBiotech)
Katarzyna Jasieniecka-Gazarkiewicz,,
- Department of Biotechnology
, Antoni Banaś (IFB / IB / DBiotech)
Antoni Banaś,,
- Department of Biotechnology
Journal seriesPlanta, ISSN 0032-0935, e-ISSN 1432-2048, (N/A 100 pkt)
Issue year2019
Vol250
No5
Pages1655-1670
Publication size in sheets0.75
Keywords in EnglishFalse flax, acyl-CoA, lysophosphatidylcholine acyltransferases, phosphatidylcholine remodelling, seed lipids, TAG biosynthesis, triacylglycerol
ASJC Classification1110 Plant Science; 1311 Genetics
DOIDOI:10.1007/s00425-019-03248-6
URL https://doi.org/10.1007/s00425-019-03248-6
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 28-01-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.203; WoS Impact Factor: 2018 = 3.06 (2) - 2018=3.408 (5)
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