Simultaneous determination of scopolamine, hyoscyamine andanisodamine in in vitro growth media of selected Solanaceae hairyroots by CE method

Szymon Dziomba , Teresa Łepek , Zbigniew Jaremicz , Maria Łuczkiewicz , Adam Prahl , Piotr Kowalski

Abstract

An electrophoretic method for fast separation of three tropane alkaloids (hyoscyamine, anisodamine and scopolamine) was presented. The substances were complete resolved in less than one minute due to utilization of relatively short capillary (20.2 cm effective length) and high voltage (25 kV). Detector probing frequency was found among the parameters that significantly affected the detection sensitivity. The performed experiments showed insufficient available probing frequency of used commercial spectrophotometric detector according to capillary electrophoresis (CE) separation potential. Under the optimized conditions the background electrolyte (BGE) was composed of 20 mM Tris, 6 mM HCl and 20 mM NaCl (pH 8.50). All analyses were carried out in fused silica capillaries of 50 μm (inner diameter) and 31.2 cm (total capillary length). Samples were injected hydrodynamically (5 s; 3.45 kPa) without any sample preparation step and separation was performed at 25 kV. The elaborated method was applied in plant cultures growth media analysis after incubation with hairy roots of selected Solanaceae species. The performed experiments proved the usefulness of CE in quality control of biotechnological processes.
Author Szymon Dziomba
Szymon Dziomba,,
-
, Teresa Łepek PChP
Teresa Łepek,,
- Laboratory of Chemistry of Biopolymers
, Zbigniew Jaremicz
Zbigniew Jaremicz,,
-
, Maria Łuczkiewicz
Maria Łuczkiewicz,,
-
, Adam Prahl PChP
Adam Prahl,,
- Laboratory of Chemistry of Biopolymers
, Piotr Kowalski
Piotr Kowalski,,
-
Journal seriesJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, ISSN 1570-0232
Issue year2015
Vol1001
Pages17-21
Publication size in sheets0.5
Keywords in Englishanisodamine, biotechnology, capillary zone electrophoresis, hyoscyamine, scopolamine, tropane alkaloids
DOIDOI:10.1016/j.jchromb.2015.06.029
URL http://dx.doi.org/10.1016/j.jchromb.2015.06.029
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2015 = 2.687 (2) - 2015=2.729 (5)
Citation count*0
Cite
Share Share

Get link to the record
msginfo.png


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back