The choice of the anchoring protein influences the interaction of recombinant Bacillus spores with the immune system

Aurelia Piekarska , Paulina Pełka , Grażyna Peszyńska-Sularz , Alessandro Negri , Krzysztof Hinc , Michał Obuchowski , Adam Iwanicki

Abstract

The technology of display of heterologous proteins on the surface of Bacillus subtilis spores enables use of these structures as carriers of antigens for mucosal vaccination. Currently, there are no technical possibilities to predict whether a designed fusion will be efficiently displayed on the spore surface and how such recombinant spores will interact with cells of the immune system. In this study, we compared four variants of B. subtilis spores presenting a fragment of a FliD protein from Clostridium difficile in fusion with CotB, CotC, CotG or CotZ spore coat proteins. We show that these spores promote their own phagocytosis and activate both, the J774 macrophages and JAWSII dendritic cells of murine cell lines. Moreover, we used these spores for mucosal immunization of mice. We conclude that the observed effects vary with the type of displayed FliD-spore coat protein fusion and seem to be mostly independent of its abundance and localization in the spore coat structure.
Author Aurelia Piekarska (IFB)
Aurelia Piekarska,,
- Intercollegiate Faculty of Biotechnology UG
, Paulina Pełka (IFB)
Paulina Pełka,,
- Intercollegiate Faculty of Biotechnology UG
, Grażyna Peszyńska-Sularz
Grażyna Peszyńska-Sularz,,
-
, Alessandro Negri (IFB)
Alessandro Negri,,
- Intercollegiate Faculty of Biotechnology UG
, Krzysztof Hinc (IFB / DMedB)
Krzysztof Hinc,,
- Department of Medical Biotechnology
, Michał Obuchowski (IFB / DMedB)
Michał Obuchowski,,
- Department of Medical Biotechnology
, Adam Iwanicki (IFB / DMedB)
Adam Iwanicki,,
- Department of Medical Biotechnology
Journal seriesActa Biochimica Polonica, ISSN 0001-527X, e-ISSN 1734-154X, (A 15 pkt)
Issue year2017
Vol64
No2
Pages239-244
Publication size in sheets0.5
Keywords in EnglishBacillus subtilis, spore display, APCs, FliD, Clostridium difficile, immune response
DOIDOI:10.18388/abp.2016_1315
URL http://www.actabp.pl/pdf/2_2017/2016_1315.pdf
Languageen angielski
LicenseJournal (articles only); published final; Other open licence; with publication
Score (nominal)15
ScoreMinisterial score = 15.0, 26-02-2018, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 26-02-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.159 (2) - 2016=1.491 (5)
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