The choice of the anchoring protein influences the interaction of recombinant Bacillus spores with the immune system
Aurelia Piekarska , Paulina Pełka , Grażyna Peszyńska-Sularz , Alessandro Negri , Krzysztof Hinc , Michał Obuchowski , Adam Iwanicki
AbstractThe technology of display of heterologous proteins on the surface of Bacillus subtilis spores enables use of these structures as carriers of antigens for mucosal vaccination. Currently, there are no technical possibilities to predict whether a designed fusion will be efficiently displayed on the spore surface and how such recombinant spores will interact with cells of the immune system. In this study, we compared four variants of B. subtilis spores presenting a fragment of a FliD protein from Clostridium difficile in fusion with CotB, CotC, CotG or CotZ spore coat proteins. We show that these spores promote their own phagocytosis and activate both, the J774 macrophages and JAWSII dendritic cells of murine cell lines. Moreover, we used these spores for mucosal immunization of mice. We conclude that the observed effects vary with the type of displayed FliD-spore coat protein fusion and seem to be mostly independent of its abundance and localization in the spore coat structure.
|Journal series||Acta Biochimica Polonica, ISSN 0001-527X, e-ISSN 1734-154X, (A 15 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Bacillus subtilis, spore display, APCs, FliD, Clostridium difficile, immune response|
|License||Journal (articles only); published final; ; with publication|
|Score|| = 15.0, ArticleFromJournal|
= 15.0, ArticleFromJournal
|Publication indicators||: 2017 = 1.239 (2) - 2017=1.541 (5)|
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