The choice of the anchoring protein influences the interaction of recombinant Bacillus spores with the immune system

Aurelia Piekarska , Paulina Pełka , Grażyna Peszyńska-Sularz , Alessandro Negri , Krzysztof Hinc , Michał Obuchowski , Adam Iwanicki


The technology of display of heterologous proteins on the surface of Bacillus subtilis spores enables use of these structures as carriers of antigens for mucosal vaccination. Currently, there are no technical possibilities to predict whether a designed fusion will be efficiently displayed on the spore surface and how such recombinant spores will interact with cells of the immune system. In this study, we compared four variants of B. subtilis spores presenting a fragment of a FliD protein from Clostridium difficile in fusion with CotB, CotC, CotG or CotZ spore coat proteins. We show that these spores promote their own phagocytosis and activate both, the J774 macrophages and JAWSII dendritic cells of murine cell lines. Moreover, we used these spores for mucosal immunization of mice. We conclude that the observed effects vary with the type of displayed FliD-spore coat protein fusion and seem to be mostly independent of its abundance and localization in the spore coat structure.
Author Aurelia Piekarska MWB UG i GUMed
Aurelia Piekarska,,
- Intercollegiate Faculty of Biotechnology UG
, Paulina Pełka MWB UG i GUMed
Paulina Pełka,,
- Intercollegiate Faculty of Biotechnology UG
, Grażyna Peszyńska-Sularz
Grażyna Peszyńska-Sularz,,
, Alessandro Negri MWB UG i GUMed
Alessandro Negri,,
- Intercollegiate Faculty of Biotechnology UG
, Krzysztof Hinc KBM
Krzysztof Hinc,,
- Department of Medical Biotechnology
, Michał Obuchowski KBM
Michał Obuchowski,,
- Department of Medical Biotechnology
, Adam Iwanicki KBM
Adam Iwanicki,,
- Department of Medical Biotechnology
Journal seriesActa Biochimica Polonica, ISSN 0001-527X, e-ISSN 1734-154X
Issue year2017
Publication size in sheets0.5
Keywords in EnglishBacillus subtilis, spore display, APCs, FliD, Clostridium difficile, immune response
Languageen angielski
LicenseJournal (articles only); published final; Other open licence; with publication
Score (nominal)15
ScoreMinisterial score = 15.0, 26-02-2018, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 26-02-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.159 (2) - 2016=1.491 (5)
Citation count*0
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.