Overactivity of alternative pathway convertases in patients with complement-mediated renal diseases

Marloes A. H. M. Michels , Nicole C. A. J. van de Kar , Marcin Okrój , Anna M. Blom , Sanne A. W. van Kraaij , Elena B. Volokhina , Lambertus P. W. J. van den Heuvel

Abstract

Overactivation of the alternative pathway of the complement system is associated with the renal diseases atypical hemolytic uremic syndrome (aHUS) and C3 glomerulopathy (C3G). C3 nephritic factors (C3NeF) play an important role in C3G pathogenesis by stabilizing the key enzymatic complex of complement, the C3 convertase. However, the reliability of assays detecting these autoantibodies is limited. Therefore, in this study, we validated and optimized a prototype hemolytic method for robust detection and characterization of factors causing convertase overactivity in large patient cohorts. The assay assesses convertase activity directly in the physiological milieu of serum and therefore is not restricted to detection of stabilizing autoantibodies such as C3NeF but may also reveal genetic variants resulting in prolonged convertase activity. We first defined clear cutoff values based on convertase activity in healthy controls. Next, we evaluated 27 C3G patient samples and found 16 positive for prolonged convertase activity, indicating the presence of factors influencing convertase stability. In three patients, the overactive convertase profile was persistent over disease course while in another patient the increased stability normalized in remission. In all these four patients, the convertase-stabilizing activity resided in the purified immunoglobulin (Ig) fraction, demonstrating the autoantibody nature. By contrast, the Igs of a familial aHUS patient carrying the complement factor B mutation p.Lys323Glu did not reveal convertase stabilization. However, in serum prolonged convertase activity was observed and segregated with the mutation in both affected and unaffected family members. In conclusion, we present a robust and reliable method for the detection, characterization, and evaluation over time of factors prolonging convertase activity (C3NeF or certain mutations) in patient cohorts. This assay may provide new insights in disease pathogenesis and may contribute to the development of more personalized treatment strategies.
Author Marloes A. H. M. Michels
Marloes A. H. M. Michels,,
-
, Nicole C. A. J. van de Kar
Nicole C. A. J. van de Kar,,
-
, Marcin Okrój (IFB / DMedB)
Marcin Okrój,,
- Department of Medical Biotechnology
, Anna M. Blom
Anna M. Blom,,
-
, Sanne A. W. van Kraaij
Sanne A. W. van Kraaij,,
-
, Elena B. Volokhina
Elena B. Volokhina,,
-
, Lambertus P. W. J. van den Heuvel
Lambertus P. W. J. van den Heuvel,,
-
Journal seriesFrontiers in Immunology, ISSN 1664-3224, (A 35 pkt)
Issue year2018
Vol9
Pages1-13
Publication size in sheets0.6
Keywords in Englishcomplement system, alternative pathway, convertase, C3 nephritic factor, C3 glomerulopathy, atypical hemolytic uremic syndrome, complement factor B mutation
DOIDOI:10.3389/fimmu.2018.00612
URL https://doi.org/10.3389/fimmu.2018.00612
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)35
ScoreMinisterial score = 35.0, 30-05-2018, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 30-05-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 6.429 (2) - 2016=5.849 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back