Wolbachia and Cardinium infection found in threatened unionid species: a new concern for conservation of freshwater mussels?

Monika Mioduchowska , Katarzyna Zając , Tadeusz Zając , Jerzy Sell

Abstract

Endosymbiotic bacterial species that manipulate host biology, reproduction and mitochondrial genetic diversity have been identified in many metazoans, especially terrestrial arthropods. Until now, the hypothesis that Wolbachia or other bacte-rial endosymbiont might be absent in mollusks has remained unexplored. We present here preliminary data on bacterial communities in a freshwater mussel Unio crassus—species with doubly uniparental inheritance of mtDNA (DUI). Next generation sequencing of 16S rRNA bacterial gene fragment allowed to identify endosymbiotic Cardinium and sequences that were classified to the order Rickettsiales. Finally, we discovered Wolbachia and confirmed Cardinium infection of Unio crassus using bacterial species-specific primers. Discovering Wolbachia and Cardinium infections in Unio crassus opens new opportunities of further investigations in the second largest animal phylum on Earth, very diversified phylogenetically, widespread geographically and inhabiting many environs, including freshwater, inhabited by the most threatened molluscan species. Considering the problems caused by endosymbionts identified in arthropods, the presence of endosymbiotic factor implies possibility of their influence on taxonomy of threatened unionids, on the results of studies of genetic diversity and proper conservation planning.
Author Monika Mioduchowska (FB/DGB)
Monika Mioduchowska,,
- Katedra Genetyki i Biosystematyki
, Katarzyna Zając
Katarzyna Zając,,
-
, Tadeusz Zając
Tadeusz Zając,,
-
, Jerzy Sell (FB/DGB)
Jerzy Sell,,
- Katedra Genetyki i Biosystematyki
Journal seriesConservation Genetics, ISSN 1566-0621, e-ISSN 1572-9737, (N/A 70 pkt)
Issue year2020
Vol21
No2
Pages381-386
Publication size in sheets0.50
Keywords in EnglishUnio crassus, bacterial endosymbiont, next generation sequencing, microbiome, 16S rRNA
ASJC Classification1105 Ecology, Evolution, Behavior and Systematics; 1311 Genetics
DOIDOI:10.1007/s10592-020-01255-9
URL https://doi.org/10.1007/s10592-020-01255-9
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 16-04-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.894; WoS Impact Factor: 2018 = 2.283 (2) - 2018=2.188 (5)
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