The sequence dependence of photoinduced single strand break in 5-Bromo-2'-deoxyuridine labeled DNA supports that electron transfer is responsible for the damage

Kinga Westphal , Samanta Makurat , Janusz Rak

Abstract

The UVB irradiation of DNA labeled with 5-bromo-2′-deoxyuridine (BrdU) leads to single-strand breaks (SSBs) as a major photochemical damage. Some time ago, we demonstrated that SSB is a secondary damage forming due to thermal dissociation of 2′-deoxyribonolactone generated photochemically in DNA labeled with BrdU. For the first time, we study here the variation of the yield of UVB generated SSBs with the alteration of 3′-neighbor nucleobase of electron donor (2′-deoxyguanine (dG)) and acceptor (excited BrdU) in double-stranded DNA. We showed that the experimental damage yields can be explained by the calculated ionization potentials of dG and electron affinities of excited BrdU via a kinetic scheme based on the Marcus model of electron transfer (ET). Hence, our studies on the sequence dependence of photochemical damage in DNA labeled with BrdU constitute a further argument that photochemically generated SSBs occur as a result of long-range ET.
Author Kinga Westphal PSB
Kinga Westphal ,,
- Laboratory of Biological Sensitizers
, Samanta Makurat PSB
Samanta Makurat,,
- Laboratory of Biological Sensitizers
, Janusz Rak PSB
Janusz Rak,,
- Laboratory of Biological Sensitizers
Other language title versions
Journal seriesJournal of Physical Chemistry B, ISSN 1520-6106
Issue year2017
Vol121
No39
Pages9169-9174
Publication size in sheets0.5
DOIDOI:10.1021/acs.jpcb.7b07338
URL http://pubs.acs.org/doi/pdf/10.1021/acs.jpcb.7b07338
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.177 (2) - 2016=3.177 (5)
Citation count*0
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